J Korean Ophthalmol Soc.  2018 Nov;59(11):1056-1061. 10.3341/jkos.2018.59.11.1056.

Effects of Valproic Acid on the Survival of Human Tennon's Capsule Fibroblasts

Affiliations
  • 1Department of Ophthalmology, Daegu Catholic University School of Medicine, Daegu, Korea. jwkim@cu.ac.kr

Abstract

PURPOSE
To investigate the effects of valproic acid on the survival of cultured human Tenon's capsule fibroblasts (HTFBs).
METHODS
Primary cultured HTFBs were exposed to 0, 0.25, 0.5, and 1.0 mM valproic acid with or without 0, 1.0, 2.5 µg/mL mitomycin C, and incubated for 5 days. Cell survival was assessed using an MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and the degree of apoptosis was assessed by flow cytometry using annexin-V/propidium iodide double staining.
RESULTS
Valproic acid decreased the survival of HTFBs in a dose-dependent manner, and survival was further decreased by adding mitomycin C to valproic acid. Both valproic acid and mitomycin C induced apoptosis of HTFBs. Valproic acid induced less apoptosis than mitomycin C.
CONCLUSIONS
Valproic acid decreased the cellular survival of HTFBs and induced apoptosis. The antiproliferative effects of valproic acid were further enhanced by the addition of mitomycin C.

Keyword

Apoptosis; Fibroblast; Flow cytometry; Mitomycin C; Valproic acid

MeSH Terms

Apoptosis
Cell Survival
Fibroblasts*
Flow Cytometry
Humans*
Mitomycin
Tenon Capsule
Valproic Acid*
Mitomycin
Valproic Acid
Full Text Links
  • JKOS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error