J Cancer Prev.  2017 Sep;22(3):195-201. 10.15430/JCP.2017.22.3.195.

Regulation of Inflammation by Sucrose Isomer, Turanose, in Raw 264.7 Cells

• Chung JY
• Kim YS
• Kim Y
• Yoo SH

• KMID: 2396169
• DOI: http://doi.org/10.15430/JCP.2017.22.3.195

Abstract

Increased sugar consumption has been proposed to be a risk factor for obesity-related metabolic disorders. The objective of this study was to investigate the anti-inflammatory effect of turanose in Raw 264.7 macrophages. Turanose (3-O-α-D-glucosyl-D-fructose), an isomer of sucrose, naturally exists in honey. For these studies, macrophages were treated with total glucose (Glu), 50% Glu/50% turanose (T50), 25% Glu/75% turanose (T75), and 100% turanose (T100), each with a total concentration of 25 mM in cell media. Expressions of inflammatory enzymes and cytokines were analyzed. Cell viability was not affected in the turanose treated groups compared to the Glu group. Lipopolysaccharide and glucose-induced nitric oxide production, protein expression of inducible nitric oxide synthase, COX-2, and superoxide dismutase 2, and mRNA expression levels of interleukin (IL)-1β and IL-18 were significantly suppressed by turanose treatment. These results demonstrate that turanose exerts anti-inflammatory effects in vitro, and possesses potential to serve therapeutic functional sweetener for testing in vivo and in clinical trials.

Keyword

Turanose; Sweetening agents; Macrophages; Inflammation

MeSH Terms

Cell Survival
Cytokines
Glucose
Honey
In Vitro Techniques
Inflammation*
Interleukin-18
Interleukins
Macrophages
Nitric Oxide
Nitric Oxide Synthase Type II
RAW 264.7 Cells*
Risk Factors
RNA, Messenger
Sucrose*
Superoxide Dismutase
Sweetening Agents
Cytokines
Glucose
Interleukin-18
Interleukins
Nitric Oxide
Nitric Oxide Synthase Type II
RNA, Messenger
Sucrose
Superoxide Dismutase
Sweetening Agents