Ann Dermatol.  1999 Oct;11(4):225-231. 10.5021/ad.1999.11.4.225.

Effects of Ultraviolet A and B Irradiation on the Transcriptional Regulation of Stromelysin-1 Gene in Human Fibroblast Cultures

• Kim BC
• Park JH
• Lee KS

• KMID: 2383028
• DOI: http://doi.org/10.5021/ad.1999.11.4.225

Abstract

BACKGROUND
Sun exposure and therapeutic irradiation have been shown to induce alterations in extracellular matrix (ECM) proteins, including elastin, glycosaminoglycan and collagens. The integrity of the connective tissue mainly depends on balanced rates of matrix synthesis and degradation of the extracellular matrix. Therefore, matrix metalloproteinases (MMPs) may be involved in ultraviolet irradiation (UVR)-induced alterations in ECM proteins.
OBJECTIVE
To evaluate the effects of UVA as well as UVB irradiations on ST-1 gene expression in cultured human skin fibroblasts.
METHODS
After exposure of different doses of UVA and UVB on cultured human skin fibroblasts, we examined the expression of ST-1 gene by Northern blot analysis, chloramphenicol acetyltransferase (CAT) assay with CAT construct containing AP-1 binding site. Additionally, we carried out the gel mobility shift assay to investigate the effects of UVR on the DNA-binding activity of AP-1.
RESULTS
After UVR on fibroblasts, the steady-state levels of ST-1 mRNA were in-creased in response to UVA and UVB by 2.5-fold and 4.2-fold, respectively, as compared with controls. Similar results were obtained by CAT assay showing that CAT activity increased as the UVA and UVB doses increased. Furthermore, gel mobility shift assay demonstrated that both UVA and UVB increased AP-1 DNA binding complexes.
CONCLUSION
UVB as well as UVA up-regulated ST-1 gene expression at transcriptional levels in vitro. We speculate that modulation of MMPs, including ST-1, gene expression by UVR may contribute to the connective tissue damage related to photoaging and other photocutaneous disorders.

Keyword

UVA; UVB; ST-1; In vitro

MeSH Terms

Animals
Binding Sites
Blotting, Northern
Cats
Chloramphenicol O-Acetyltransferase
Collagen
Connective Tissue
DNA
Elastin
Electrophoretic Mobility Shift Assay
Extracellular Matrix
Fibroblasts*
Gene Expression
Humans*
In Vitro Techniques
Matrix Metalloproteinases
RNA, Messenger
Skin
Solar System
Transcription Factor AP-1
Chloramphenicol O-Acetyltransferase
Collagen
DNA
Elastin
Matrix Metalloproteinases
RNA, Messenger
Transcription Factor AP-1