Biomol Ther.  2015 Nov;23(6):557-563. 10.4062/biomolther.2015.054.

Diphlorethohydroxycarmalol Suppresses Ultraviolet B-Induced Matrix Metalloproteinases via Inhibition of JNK and ERK Signaling in Human Keratinocytes

• Piao MJ
• Susara Ruwan Kumara MH
• Kim KC
• Kang KA
• Kang HK
• Lee NH
• Hyun JW

• KMID: 2380307
• DOI: http://doi.org/10.4062/biomolther.2015.054

Abstract

Skin aging is the most readily observable process involved in human aging. Ultraviolet B (UVB) radiation causes photo-oxidation via generation of reactive oxygen species (ROS), thereby damaging the nucleus and cytoplasm of skin cells and ultimately leading to cell death. Recent studies have shown that high levels of solar UVB irradiation induce the synthesis of matrix metalloproteinases (MMPs) in skin fibroblasts, causing photo-aging and tumor progression. The MMP family is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes such as arthritis and metastasis. We investigated the effect of diphlorethohydroxycarmalol (DPHC) against damage induced by UVB radiation in human skin keratinocytes. In UVB-irradiated cells, DPHC significantly reduced expression of MMP mRNA and protein, as well as activation of MMPs. Furthermore, DPHC reduced phosphorylation of ERK and JNK, which act upstream of c-Fos and c-Jun, respectively; consequently, DPHC inhibited the expression of c-Fos and c-Jun, which are key components of activator protein-1 (AP-1, up-regulator of MMPs). Additionally, DPHC abolished the DNA-binding activity of AP-1, and thereby prevented AP-1-mediated transcriptional activation. These data demonstrate that by inactivating ERK and JNK, DPHC inhibits induction of MMPs triggered by UVB radiation.

Keyword

Diphlorethohydroxycarmalol; Human skin keratinocytes; Matrix metalloproteinases; Photo-aging; Ultraviolet B

MeSH Terms

Aging
Arthritis
Cell Death
Cytoplasm
Embryonic Development
Extracellular Matrix
Female
Fibroblasts
Humans*
Keratinocytes*
Matrix Metalloproteinases*
Neoplasm Metastasis
Phosphorylation
Physiological Processes
Pregnancy
Reactive Oxygen Species
Reproduction
RNA, Messenger
Skin
Skin Aging
Transcription Factor AP-1
Transcriptional Activation
Matrix Metalloproteinases
RNA, Messenger
Reactive Oxygen Species
Transcription Factor AP-1