Yonsei Med J.  2016 May;57(3):761-768. 10.3349/ymj.2016.57.3.761.

STAT3 and ERK Signaling Pathways Are Implicated in the Invasion Activity by Oncostatin M through Induction of Matrix Metalloproteinases 2 and 9

• Ko HS
• Park BJ
• Choi SK
• Kang HK
• Kim A
• Kim HS
• Park IY
• Shin JC

• KMID: 2374099
• DOI: http://doi.org/10.3349/ymj.2016.57.3.761

Abstract

PURPOSE
Our previous studies have shown that oncostatin M (OSM) promotes trophoblast invasion activity through increased enzyme activity of matrix metalloproteinase (MMP)-2 and -9. We further investigated OSM-induced intracellular signaling mechanisms associated with these events in the immortalized human trophoblast cell line HTR8/SVneo.
MATERIALS AND METHODS
We investigated the effects of OSM on RNA and protein expression of MMP-2 and -9 in the first-trimester extravillous trophoblast cell line (HTR8/SVneo) via Western blot. The selective signal transducer and activator of transcription (STAT)3 inhibitor, stattic, STAT3 siRNA, and extracellular signal-regulated kinase (ERK) siRNA were used to investigate STAT3 and ERK activation by OSM. The effects of STAT3 and ERK inhibitors on OSM-induced enzymatic activities of MMP-2 and -9 and invasion activity were further determined via Western blot and gelatin zymography.
RESULTS
OSM-induced MMP-2 and -9 protein expression was significantly suppressed by STAT3 inhibition with stattic and STAT3 siRNA silencing, whereas the ERK1/2 inhibitor (U0126) and ERK silencing significantly suppressed OSM-induced MMP-2 protein expression. OSM-induced MMP-2 and MMP-9 enzymatic activities were significantly decreased by stattic pretreatment. The increased invasion activity induced by OSM was significantly suppressed by STAT3 and ERK1/2 inhibition, though to a greater extent by STAT3 inhibition.
CONCLUSION
Both STAT3 and ERK signaling pathways are involved in OSM-induced invasion activity of HTR8/SVneo cells. Activation of STAT3 appears to be critical for the OSM-mediated increase in invasiveness of HTR8/SVneo cells.

Keyword

Oncostatin M; trophoblasts; signaling pathway; signal transducer and activator of transcription (STAT)3; extracellular signal-regulated kinases (ERK)1/2

MeSH Terms

Blotting, Western
Cell Movement/*drug effects
Cell Proliferation/*drug effects
Extracellular Signal-Regulated MAP Kinases/metabolism
Humans
Matrix Metalloproteinase 2/genetics/*metabolism
Matrix Metalloproteinase 9/genetics/*metabolism
Oncostatin M/genetics/*metabolism
Phosphorylation/drug effects
RNA, Messenger/metabolism
RNA, Small Interfering
STAT3 Transcription Factor/*metabolism
Signal Transduction/*drug effects
Extracellular Signal-Regulated MAP Kinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Oncostatin M
RNA, Messenger
RNA, Small Interfering
STAT3 Transcription Factor

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