J Korean Gastric Cancer Assoc.  2005 Dec;5(4):260-265.

Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability

• Lee JH
• Cho YG
• Song JW
• Park CH
• Nam SW
• Lee SH
• Yoo NJ
• Lee JY
• Park WS

Abstract

PURPOSE
The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers.
MATERIALS AND METHODS
The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene.
RESULTS
We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases.
CONCLUSION
These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.

Keyword

Gastric cancer; Microsatellite instability; Mutation; Cell cycle

MeSH Terms

Cell Cycle
Cell Cycle Checkpoints
DNA Damage
Frameshift Mutation
Microdissection
Microsatellite Instability*
Microsatellite Repeats*
Protein Kinases
Stomach Neoplasms*
Protein Kinases