J Korean Ophthalmol Soc.  1999 Jun;40(6):1496-1502.

The Effects of Steroids and Nonsteroidal Antiinflammatory Agents on Proliferation of Human Ocular Fibroblast

• Lee JS
• Jung W
• Choi YR
• Kim YS

Abstract

Corticosteroid, 5-fluorouracil and Mitomycin C(MMC) have been used to suppress proliferation of subconjunctival connective tissue. However, the various complication and undesirable side effects associated with the use of these drugs have prompted the search for alternative drugs with less toxicity. In present study, I have evaluated the effect of corticosteroid(dexamethsone sodium phophate), cyclooxygenase inhibitor(diclofenac sodium) and lipoxygenase inhibitor(nordihydroguaratic acid, NDGA) to inhibit human pterygial fibroblast proliferation in tissue culture. Human pterygial fibroblast were cultured and exposed to various concentration of each drug for 1, 2 and 3 days. Cell viability was measured using MTT assay. There was no different in antiproliferative activity between nonsteroidal antiinflammatory agents(NSAIDs) and steroid. Dexamethasone caused inhibition of proliferation with LD 50 of 0.5mM by day 2, NDGA of 0.5mM by day 3 and diclofenac sodium of 1.0mM by day 3. These results suggest that NSAIDs may be useful as both antiproliferative and less toxicity agents.

Keyword

Dexamethasone; Fibroblast; NSAIDs

MeSH Terms

Anti-Inflammatory Agents, Non-Steroidal*
Cell Survival
Connective Tissue
Dexamethasone
Diclofenac
Fibroblasts*
Fluorouracil
Humans*
Lipoxygenase
Mitomycin
Prostaglandin-Endoperoxide Synthases
Sodium
Steroids*
Anti-Inflammatory Agents, Non-Steroidal
Dexamethasone
Diclofenac
Fluorouracil
Lipoxygenase
Mitomycin
Prostaglandin-Endoperoxide Synthases
Sodium
Steroids