The Effects of Ascorbic Acids on the Proliferation of the Human & Rabbit Tenon's Fibroblasts

Lee, S Y; Ahn, J H; Lew, H M

Abstract

Although ascorbic acids have been known to play a role in the hydroxylation of procollagen in collagen synthesis, it can also inhibit the proliferation of fibroblasts at high concentrations. The time-and dose-related effects of ascorbic acids in proliferation of human and rabbit Tenon's fibroblasts were studied. Fibroblasts were incubated in different concentrations of ascorbic acid at 0.75mM, 1.00mM, 1.25mM, 1, 50mM, and 2.00mM and evaluated on days 1, 2, and 5 after exposure to ascorbic acid for 24 hours. To evaluate the effects of different concentration of ascorbic acids on fibroblast proliferation, cell density was quantified by hemacytometer. In conclusion, ascorbic acid was a potent inhibitor of fibroblast proliferation, the effective concentrations of ascorbic acid were[average ID5 0] 1.14, 1.53, 1.72mM in human fibroblast, and 1.59, 1.16, 1.54mM in rabbit on days 1, 2, and 5. The effective concentration of ascorbic acid in humans was similar to the normal human concentration of ascorbic acid in aqueous humor on day 1, and the concentration of ascorbic acid in rabbits was similar to the normal rabbit aqueous humor on day 2. This study shows that ascorbic acid inhibits cell proliferation in concentration of 1.14mM or more in humans and 1.16mM or more in rabbits, future clinical studies may show that these agents reduce bleb failure following glaucoma filtration surgery.