Mycobiology.  2015 Sep;43(3):319-326. 10.5941/MYCO.2015.43.3.319.

Methyl 9-Oxo-(10E,12E)-octadecadienoate Isolated from Fomes fomentarius Attenuates Lipopolysaccharide-Induced Inflammatory Response by Blocking Phosphorylation of STAT3 in Murine Macrophages

• Choe JH
• Yi YJ
• Lee MS
• Seo DW
• Yun BS
• Lee SM

• KMID: 2312829
• DOI: http://doi.org/10.5941/MYCO.2015.43.3.319

Abstract

Fomes fomentarius is a fungus of the Polyporaceae family and is used in traditional oriental therapies. Although the anti-inflammatory activities of this species have been previously reported, the identity of the bioactive compounds responsible for this activity remains unknown. Here, we investigated whether methyl 9-oxo-(10E,12E)-octadecadienoate (FF-8) purified from F. fomentarius exerts anti-inflammatory activity in murine macrophages stimulated with lipopolysaccharide (LPS). FF-8 suppressed secretion of nitric oxide (NO) and prostaglandin E2 through downregulation of inducible NO synthase and cyclooxygenase-2 expression induced by LPS. In addition, pretreatment of cells with FF-8 led to a reduction in levels of secreted inflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6 in macrophages stimulated with LPS. Conversely, FF-8 did not affect nuclear factor kappaB, p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase pathways. Instead, FF-8 specifically interfered with signal transducer and activator of transcription 3 (STAT3) phosphorylation induced by LPS. Collectively, this study demonstrated that FF-8 purified from F. fomentarius suppresses inflammatory responses in macrophages stimulated with LPS by inhibiting STAT3 activation. Further studies will be required to elucidate the anti-inflammatory effect of FF-8 in vivo.

Keyword

Anti-inflammatory effect; Fomes fomentarius; Macrophages; STAT3

MeSH Terms

Coriolaceae*
Cyclooxygenase 2
Cytokines
Dinoprostone
Down-Regulation
Fungi
Humans
Interleukin-6
Macrophages*
Nitric Oxide
Nitric Oxide Synthase
Phosphorylation*
Phosphotransferases
Polyporaceae
STAT3 Transcription Factor
Tumor Necrosis Factor-alpha
Cyclooxygenase 2
Cytokines
Dinoprostone
Interleukin-6
Nitric Oxide
Nitric Oxide Synthase
Phosphotransferases
STAT3 Transcription Factor
Tumor Necrosis Factor-alpha