Korean J Neurogastroenterol Motil.
2006 Dec;12(2):151-156.
Modulation of Pacemaker Ca2+ Activity by Serotonin in the Cultured Interstitial Cells of the Cajal Clusters Isolated from Mouse Small Intestine
- Affiliations
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- 1Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea. plrhee@smc.samsung.co.kr
- 2Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
- 3Department of Physiology and Biophysics, Seoul National University College of Medicine, Seoul, Republic of Korea.
Abstract
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BACKGROUND/AIMS: Interstitial cells of Cajal (ICCs) are considered to be pacemaker cells in the gastrointestinal tracts and Ca2+ oscillation in ICCs is intimately linked to the pacemaker function. Although serotonin is one of most importance substance involved in gastrointestinal motility, it's effects on the ICCs are not known. We wanted to study the effect of serotonin on Ca2+ oscillation in ICCs.
METHODS
Using cultured cell clusters isolated from the mouse ileum, we measured spontaneous Ca2+ oscillation as an index of pacemaker activity in the ICCs. The cultured cell clusters showed spontaneous contraction and they contained c-Kit immunopositive cells. We visualized the changes in intracellular Ca2 concentration in the ICCs within the cultured cell clusters that were loaded with a fluorescent Ca2 indicator, fura-2.
RESULTS
There was no significant change in the fluorescence emission intensity and spike frequency between the baseline and after the addition of TTX. The addition of serotonin in the buffer solution caused an increase in the fluorescence emission intensity and the spike frequency per minute of Ca2+ oscillations. The addition of serotonin in the presence of TTX caused increases in the spike frequency per minute of the calcium oscillation.
CONCLUSIONS
These results suggest that serotonin may modulate the pacemaker Ca2+ activity in ICCs by the TTX-resistant, direct serotonergic pathway.
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