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Effect of Sphingosine-1-Phosphate on Intracellular Free Ca2+ in Cat Esophageal Smooth Muscle Cells

Lee DK, Min YS, Yoo SS, Shim HS, Park SY, Sohn UD

A comprehensive collection of proteins senses local changes in intracellular Ca²⁺ concentrations ([Ca²⁺](i) and transduces these signals into responses to agonists. In the present study, we examined the effect of...
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Oxytocin produces thermal analgesia via vasopressin-1a receptor by modulating TRPV1 and potassium conductance in the dorsal root ganglion neurons

Han RT, Kim HB, Kim YB, Choi K, Park GY, Lee PR, Lee J, Kim HY, Park CK, Kang Y, Oh SB, Na HS

Recent studies have provided several lines of evidence that peripheral administration of oxytocin induces analgesia in human and rodents. However, the exact underlying mechanism of analgesia still remains elusive. In...
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Lipid emulsion inhibits vasodilation induced by a toxic dose of bupivacaine by suppressing bupivacaine-induced PKC and CPI-17 dephosphorylation but has no effect on vasodilation induced by a toxic dose of mepivacaine

Cho H, Ok SH, Kwon SC, Lee SH, Baik J, Kang S, Oh J, Sohn JT

BACKGROUND: The goal of this in vitro study was to investigate the effect of lipid emulsion on vasodilation caused by toxic doses of bupivacaine and mepivacaine during contraction induced by...
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Effects of Estrogen on Intracellular Calcium-Related T-Lymphocyte Function

Kim YY, Kim H, Ku SY, Suh CS, Kim SH, Choi YM, Kim SJ

Regulation of immune cell function is an important in the field of hormone-related tissue engineering and regenerative medicine. In this sense, hormonal regulation of immune cell function is a critical...
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A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca2+ Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat

Lee JH, Ha JM, Leem CH

Fura-2 analogs are ratiometric fluoroprobes that are widely used for the quantitative measurement of [Ca2+]. However, the dye usage is intrinsically limited, as the dyes require ultraviolet (UV) excitation, which...
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Mepivacaine-induced intracellular calcium increase appears to be mediated primarily by calcium influx in rat aorta without endothelium

Ok SH, Kwon SC, Kang S, Choi MJ, Sohn JT

BACKGROUND: Mepivacaine induces contraction or decreased blood flow both in vivo and in vitro. Vasoconstriction is associated with an increase in the intracellular calcium concentration ([Ca2+]i). However, the mechanism responsible...
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Protective Effect of Metformin on Gentamicin-Induced Vestibulotoxicity in Rat Primary Cell Culture

Lee JY, Lee SH, Chang JW, Song JJ, Jung HH, Im GJ

OBJECTIVES: One of the antidiabetic drugs, metformin, have shown that it prevented oxidative stress-induced death in several cell types through a mechanism involving the opening of the permeability transition pore...
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Effects of NaOCl on Neuronal Excitability and Intracellular Calcium Concentration in Rat Spinal Substantia Gelatinosa Neurons

Lee HI, Park AR, Chun SW

  • KMID: 1431123
  • Int J Oral Biol.
  • 2013 Mar;38(1):5-12.
Recent studies indicate that reactive oxygen species (ROS) can act as modulators of neuronal activity, and are critically involved in persistent pain primarily through spinal mechanisms. In this study, we...
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Effects of glucosamine on Ca2+ signaling and K+ channel currents in T lymphocytes

Suh EY, Pang B, Kim SJ, Kim WK

  • KMID: 1458431
  • Korean J Med.
  • 2010 Nov;79(5):536-542.
BACKGROUND/AIMS: Glucosamine is widely taken as a functional food, and some studies reported its anti-inflammatory effects. K+ channels and intracellular signal play important roles in the activation of immune cells...
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Oxidized Low-density Lipoprotein- and Lysophosphatidylcholine-induced Ca2+ Mobilization in Human Endothelial Cells

Kim MY, Liang GH, Kim JA, Choi SS, Choi S, Suh SH

The effects of oxidized low-density lipoprotein (OxLDL) and its major lipid constituent lysophosphatidylcholine (LPC) on Ca2+ entry were investigated in cultured human umbilical endothelial cells (HUVECs) using fura-2 fluorescence and...
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PKC-Independent Stimulation of Cardiac Na+/Ca2+ Exchanger by Staurosporine

Kang TM

[Ca2+]i transients by reverse mode of cardiac Na+/Ca2+ exchanger (NCX1) were recorded in fura-2 loaded BHK cells with stable expression of NCX1. Repeated stimulation of reverse NCX1 produced a long-lasting...
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Modulation of Pacemaker Ca2+ Activity by Serotonin in the Cultured Interstitial Cells of the Cajal Clusters Isolated from Mouse Small Intestine

Sinn DH, Rhee PL, Son HJ, Kim JJ, Lee JY, So IS

  • KMID: 2307492
  • Korean J Neurogastroenterol Motil.
  • 2006 Dec;12(2):151-156.
BACKGROUND/AIMS: Interstitial cells of Cajal (ICCs) are considered to be pacemaker cells in the gastrointestinal tracts and Ca2+ oscillation in ICCs is intimately linked to the pacemaker function. Although serotonin...
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The Effects of DTBNP on Intracellular Ca2+ Signaling in Cultured Bovine Aortic Endothelial Cells

Park SJ, Kim BJ, Zhu MH, So I, Kim KW

  • KMID: 2071617
  • Korean J Physiol Pharmacol.
  • 2005 Dec;9(6):341-346.
The mechanism underlying oxidant-induced intracellular Ca2+ ([Ca2+]i) increase was studied in cultured bovine aortic endothelial cells (BAECs) using fura-2 AM. In the presence of 2 mM extracellular Ca2+, the application...
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Buffering Contribution of Mitochondria to the [Ca2+]i Increase by Ca2+ Influx through Background Nonselective Cation Channels in Rabbit Aortic Endothelial Cells

Kim YC, Lee SJ, Kim KW

  • KMID: 2285307
  • Korean J Physiol Pharmacol.
  • 2005 Feb;9(1):29-35.
To prove the buffering contribution of mitochondria to the increase of intracellular Ca2+ level ([Ca2+]i) via background nonselective cation channel (background NSCC), we examined whether inhibition of mitochondria by protonophore...
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Mechanism of Acetylcholine-induced Endothelium-dependent Relaxation in the Rabbit Carotid Artery by M3-receptor Activation

Song YJ, Kwon SC

  • KMID: 1611489
  • Korean J Physiol Pharmacol.
  • 2004 Dec;8(6):313-317.
The present study were designed to characterize the action mechanisms of acetylcholine (ACh) -induced endothelium-dependent relaxation in arteries precontracted with high K (70 mM). For this, we simultaneously measured both...
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Effects of Ca2+ Channel Blockers on Expression of L-type Voltage Dependent Ca2+ Channels in Vascular Smooth Muscle Cells Obtained from the Aorta of Diabetic Rats

Yoo HJ

  • KMID: 2338249
  • J Korean Geriatr Soc.
  • 2003 Jun;7(2):95-100.
BACKGROUND: The proliferation of vascular smooth muscle cells(VSMCs) is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage...
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Mechanism of Glutamate-induced[Ca2+]i Increase in Substantia Gelatinosa Neurons of Juvenile Rats

Jung SJ, Choi JS, Kwak J, Kim J, Kim JW, Kim SJ

  • KMID: 2285271
  • Korean J Physiol Pharmacol.
  • 2003 Apr;7(2):53-58.
The glutamate receptors (GluRs) are key receptors for modulatory synaptic events in the central nervous system. It has been reported that glutamate increases the intracellularCa (2+) concentration ([Ca2+]i) and induces...
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Effects of Reactive Oxygen Species on ATP-induced intracellular Ca2+ Activity in Osteoblasts

Kim JH, Kwon TD, Nam SH, Suh CK, Hong YW

BACKGORUND: The physiological activity of osteoblsts is known to be closely related to increased intracellular Ca2+ activity ([Ca2+ ]i) in osteoblasts. The cellular regulation of ([Ca2+ ]i) in osteoblasts...
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Effects of Noradrenaline on the Membrane Potential of Prostatic Neuroendocrine Cells of Rat

Kim JH, Shin SY, Uhm DY, Kim SJ

  • KMID: 1607354
  • Korean J Physiol Pharmacol.
  • 2003 Feb;7(1):47-52.
The prostate gland contains numerous neuroendocrine cells that are believed to influence the function of the prostate gland. Our recent study demonstrated the expression of both alpha 1- and alpha...
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HCG attenuates ATP induced apoptosis in cultured human luteinized granulosa cells (hLGCs)

Kim MR, Park DW, Kim YA, Cho TS, Hwang KJ, Min CK, Jo MY

  • KMID: 2262671
  • Korean J Obstet Gynecol.
  • 2002 Mar;45(3):475-483.
OBJECTIVE: To determine the effects of hCG on extracellular ATP induced apoptosis in cultured human luteinized granulosa cells (hLGCs) METHODS: The addition of various concentrations of ATP (0, 0.1, 0.25, 0.5,...
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