Tissue Eng Regen Med.  2016 Jun;13(3):270-273. 10.1007/s13770-015-9070-1.

Effects of Estrogen on Intracellular Calcium-Related T-Lymphocyte Function

• Kim YY
• Kim H
• Ku SY
• Suh CS
• Kim SH
• Choi YM
• Kim SJ

• KMID: 2361011
• DOI: http://doi.org/10.1007/s13770-015-9070-1

Abstract

Regulation of immune cell function is an important in the field of hormone-related tissue engineering and regenerative medicine. In this sense, hormonal regulation of immune cell function is a critical issue to be solved. It has been known that ovarian sex hormone play an important roles in immune function, however, little has been known whether estrogen affects T-lymphocyte function. Human Jurkat T cells were treated with estradiol (Eâ‚‚) at concentrations of 0, 10, 100, 1000 ng/mL, and calcium response was evaluated. Intracellular calcium concentrations after Fura-2 acetoxymethyl ester treatment show an increasing trend at higher Eâ‚‚ concentrations although these alterations did not reach a statistical significance. The expression of calcium channel-related gene CACNA1C did not show any significant changes according to the concentration of Eâ‚‚. Taken together, estrogen has an implication as a possible hormonal regulator of intracellular calcium release in human Jurkat T cells via non-genomic pathway. Further studies are necessary to investigate the combined effects of sex hormones and cytokines in both T- and B-lymphocytes.

Keyword

Estrogen; T-lymphocyte; Calcium channel

MeSH Terms

B-Lymphocytes
Calcium
Calcium Channels
Cytokines
Estradiol
Estrogens*
Fura-2
Gonadal Steroid Hormones
Humans
Regenerative Medicine
T-Lymphocytes*
Tissue Engineering
Calcium
Calcium Channels
Cytokines
Estradiol
Estrogens
Fura-2
Gonadal Steroid Hormones