Korean J Physiol Pharmacol.  2004 Jun;8(3):167-172.

Kinesin Superfamily KIF5 Proteins Bind to betaIII Spectrin

Affiliations
  • 1Department of Biochemistry, College of Medicine, Inje University, Busan 614-735, Korea. daehyun@ijnc.inje.ac.kr
  • 2Department of Physiology and Biophysics, College of Medicine, Inje University, Busan 614-735, Korea.
  • 3Department of Parasitology, College of Medicine, Inje University, Busan 614-735, Korea.
  • 4Department of Psychiatry, College of Medicine, Inje University, Busan 614-735, Korea.
  • 5Department of Molecular Cell Physiology Research Group, College of Medicine, Inje University, Busan 614-735, Korea.

Abstract

The kinesin proteins (KIFs) make up a large superfamily of molecular motors that transport cargo such as vesicles, protein complexes, and organelles. KIF5 is a heterotetrameric motor that conveys vesicles and plays an important role in neuronal function. Here, we used the yeast two-hybrid system to identify the neuronal protein (s) that interacts with the tail region of KIF5 and found a specific interaction with betaIII spectrin. The amino acid residues between 1394 and 1774 of betaIII spectrin were required for the interaction with KIF5C. betaIII spectrin also bound to the tail region of neuronal KIF5A and ubiquitous KIF5B but not to other kinesin family members in the yeast two-hybrid assay. In addition, these proteins showed specific interactions, confirmed by GST pull-down assay and co-immunoprecipitation. betaIII spectrin interacted with GST-KIF5 fusion proteins, but not with GST alone. An antibody to betaIII spectrin specifically co-immunoprecipitated KIF5s associated with betaIII spectrin from mouse brain extracts. These results suggest that KIF5 motor proteins transport vesicles or organelles that are coated with betaIII spectrin.

Keyword

Kinesin; Spectrin; Molecular motors; Microtubule; Adaptor proteins

MeSH Terms

Animals
Brain
Humans
Immunoprecipitation
Kinesin*
Mice
Microtubules
Neurons
Organelles
Spectrin*
Transport Vesicles
Two-Hybrid System Techniques
Kinesin
Spectrin
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