Korean J Gastroenterol.  1999 May;33(5):697-707.

Effects of Small Molecular Antioxidants on Choline-Deficient Ethionine Supple mented Diet-Induced Acute Pancreatitis in Mice

• Choi JY
• Cho YU
• Roh KH
• Kim KH

Abstract

BACKGROUND/AIMS: It has been suggested that oxygen free radicals are involved in the initiation process of acute pancreatitis. In this study, we evaluated the role of oxygen radicals and the effect of small molecular antioxidants in the development of choline-deficient ethionine supplemented (CDE) diet-induced acute pancreatitis.
METHODS
Acute necrotizing pancreatitis was induced in young female ICR mice (12.5 +/-1.9 g) by feeding CDE diet for 48 hours. Then, the effects of antioxidant (rebamipide, N-acetyl-cysteine, allopurinol,beta-carotene, and combination of alpha-tocopherol and ascor bate) were examined.
RESULTS
CDE diet resulted in a significant increase in serum amylase level and the concentration of pancreatic malondialdehyde (MDA). It also caused pancreatic edema and in creased proinflammatory cytokines such as TNF-alpha, IL-6 and IL-1 beta in serum. Treatment of rebami pide, or combination of alpha-tocopherol and ascorbate significantly decreased the CDE diet-induced pathophysiologic deterioration of pancreas. On the other hand, allopurinol, beta-carotene and N-acetyl-cysteine showed little effect.
CONCLUSIONS
These results indicate that oxygen free radicals play an important role in the development of acute pancreatitis. Antioxidants may ameliorate the CDE diet induced acute pancreatitis. Further evaluation of antioxidants such as rebamipide, combination of alpha-tocopherol and ascorbate is necessary for possible therapeutic application.

Keyword

CDE; Oxygen radical; Acute pancreatitis; Antioxidant

MeSH Terms

Allopurinol
alpha-Tocopherol
Amylases
Animals
Antioxidants*
beta Carotene
Cytokines
Diet
Edema
Ethionine*
Female
Free Radicals
Hand
Humans
Interleukin-1beta
Interleukin-6
Malondialdehyde
Mice*
Mice, Inbred ICR
Oxygen
Pancreas
Pancreatitis*
Pancreatitis, Acute Necrotizing
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Allopurinol
Amylases
Antioxidants
Cytokines
Ethionine
Free Radicals
Interleukin-1beta
Interleukin-6
Malondialdehyde
Oxygen
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
alpha-Tocopherol
beta Carotene