J Korean Soc Ther Radiol Oncol.  2004 Dec;22(4):298-306.

Proteomics of Protein Expression Profiling in Tissues with Different Radiosensitivity

• An JH
• Kim J
• Seong J

Abstract

PURPOSE: The purpose of this study was to identify Radiosensitivity of proteins in tissues with different radiosensitivity.
MATERIALS AND METHODS
C3H/HeJ mice were exposed to 10 Gy. The mice were sacrifiud 8 hrs after radiation. Their spleen and liver tissues were collected and analyzed histologicaly for apoptosis. The expressions of radiosusceptibility protein were analyzed by 2-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
RESULTS
The peak of apoptosis levels were 35.3+/-1.7% in spleen and 0.6+/-0.2% in liver at 8 hrs after radiation. Liver, radioresistant tissues, showed that the levels of ROS metabolism related to proteins such as cytochromm c, glutathione S transferase, NADH dehydrogenase, riken cDNA and peroxiredoxin VI increased after radiation. The expression of cytochrome c increased significantly in spleen and liver tissues after radiation. In spleen, radiosensitivity tissue, the identified proteins showed a significantly quantitative alteration following radiation. It was categorized as signal transduction, apoptosis, cytokine, Ca signal related protein, stress-related protein, cytoskeletal regulation, ROS metabolism, and others.
CONCLUSION
Differences of radiation-induced apoptosis by tissues specifted were coupled with the induction of related radiosensitivity and radioresistant proteins. The result suggests that apoptosis relate protein and redox proteins play important roles in this radiosusceptibility.

Keyword

Radiation; Proteomics; Apoptosis; Radiosusceptibility

MeSH Terms

Animals
Apoptosis
Cytochromes c
DNA, Complementary
Electrophoresis
Glutathione Transferase
Liver
Mass Spectrometry
Metabolism
Mice
NADH Dehydrogenase
Oxidation-Reduction
Peroxiredoxin VI
Proteomics*
Radiation Tolerance*
Signal Transduction
Spleen
Cytochromes c
DNA, Complementary
Glutathione Transferase
NADH Dehydrogenase
Peroxiredoxin VI