Korean J Urol.  2004 Jan;45(1):77-83.

Proteomic Approach for Identifying Marker Proteins in the Urinary Bladder Precancerous Conditions Induced by BBN(N-butyl-N-(4-hydroxybutyl) nitrosamine)

• Kim HJ
• Youn WY
• Myong NH
• Hwang CH
• Ryu JW
• Lee YS

Abstract

PURPOSE: Simple and noninvasive methods for the diagnosis of transitional cell carcinoma of the bladder are needed for the prevention of invasive tumor. A proteomic technology has recently been developed to facilitate protein profiling of biological mixtures. We investigated the role of this proteomic approach as a possible tool to detect the marker protein during the initiation stages on BBN-induced bladder carcinogenesis in rats.
MATERIALS AND METHODS
Ten rats group A were given 0.05% BBN in drinking water for 12 weeks. Ten rats in group B were designated as a control group and were not given BBN. Whole urinary bladders of all rats were excised at 12 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry.
RESULTS
A comparison of urinary bladder hyperplasia tissue with control tissue showed that five proteins; actin gamma2 propeptide, cytokeratin-20, proapolipoprotein, alpha2 actin(alpha-cardiac actin) and heat shock 27kDa protein 1 were over-expressed in hyperplastic tissues. Three protein; transcription factors, seminal vesicle secretory protein VI precursor and hypothetical protein RMT-7 were under-expressed in hyperplastic tissues.
CONCLUSIONS
In an animal model system, BBN-induced, urinary bladder mucosal hyperplasia resulted in an increase in five proteins and a decrease in three proteins. Of these altered proteins, CK-20 and SVS-VI seem to be important. The proteomic approach may be a simple and noninvasive method for monitoring and follow-up of bladder cancer patients. However more information is needed regarding CK-20 expression in nonmalignant urological disease and in human tumor tissue, and regarding SVS-VI expression in other organs, for clinical usage.

Keyword

Proteomics; Bladder; Precancerous conditions; Cytokeratin 20

MeSH Terms

Actins
Animals
Carcinogenesis
Carcinoma, Transitional Cell
Diagnosis
Drinking Water
Electrophoresis, Gel, Two-Dimensional
Follow-Up Studies
Hot Temperature
Humans
Hyperplasia
Keratin-20
Mass Spectrometry
Models, Animal
Precancerous Conditions*
Proteomics
Rats
Seminal Vesicles
Shock
Transcription Factors
Urinary Bladder Neoplasms
Urinary Bladder*
Urologic Diseases
Actins
Drinking Water
Keratin-20
Transcription Factors