Korean J Otorhinolaryngol-Head Neck Surg.  2009 Mar;52(3):228-231. 10.3342/kjorl-hns.2009.52.3.228.

Proteomic Analysis of the Specific Protein Expression in Nasal Polyp

• Park SC
• Koh SH
• Lee SH
• Lee JH

• KMID: 2277013
• DOI: http://doi.org/10.3342/kjorl-hns.2009.52.3.228

Abstract

BACKGROUND AND OBJECTIVES: Nasal polyp is a non-neoplastic, chronic inflammatory disease of the nasal mucosa, with its pathogenesis still not well known. We used proteomics, an emerging technique to identify new biomarkers in biological tissue, to find new potential biomarkers of the nasal polyp. MATERIALS AND METHOD: The study materials consisted of 5 specimens of nasal polyp taken from 5 patients who underwent endoscopic sinus surgery and 5 cases of normal nasal mucosa of the inferior turbinate as control. We did the proteomic profiling using matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS).
RESULTS
We detected about 500 protein spots. The selected twelve spots were analyzed. Six up-regulated proteins are annexin, keratin, actin, serpin 3, glutathion S-transferase P, and coactosins like protein. Six down-regulated proteins are two vimentins, two actins, fibrinogen, and hemoglobin subunit beta.
CONCLUSION
We identified 12 proteins expressed in the nasal polyp by using proteomics. These proteins may be helpful in understanding the mechanism of the pathogenesis of nasal polyp, and may be used as basic data.

Keyword

Nasal polyp; Proteomics

MeSH Terms

Actins
Biomarkers
Fibrinogen
Hemoglobins
Humans
Keratins
Nasal Mucosa
Nasal Polyps
Proteins
Proteomics
Turbinates
Vimentin
Actins
Fibrinogen
Hemoglobins
Keratins
Proteins
Vimentin