J Adv Prosthodont.  2013 Nov;5(4):402-408. 10.4047/jap.2013.5.4.402.

Effect of magnesium and calcium phosphate coatings on osteoblastic responses to the titanium surface

Affiliations
  • 1Dental Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 Project for School of Dentistry, Chonnam National University, Gwangju, Republic of Korea. cuidezhe@empal.com
  • 2R&D Center for Ti and Special Alloys, Gwangju Technopark, Gwangju, Republic of Korea.

Abstract

PURPOSE
The aim of this study was to evaluate the surface properties and in vitro bioactivity to osteoblasts of magnesium and magnesium-hydroxyapatite coated titanium.
MATERIALS AND METHODS
Themagnesium (Mg) and magnesium-hydroxyapatite (Mg-HA) coatings on titanium (Ti) substrates were prepared by radio frequency (RF) and direct current (DC) magnetron sputtering.The samples were divided into non-coated smooth Ti (Ti-S group), Mg coatinggroup (Ti-Mg group), and Mg-HA coating group (Ti-MgHA group).The surface properties were evaluated using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy (AFM). Cell adhesion, cell proliferation and alkaline phosphatase (ALP) activity were evaluated using MC3T3-E1 cells. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed.
RESULTS
Cross-sectional SEM images showed that Mg and Mg-HA depositionson titanium substrates were performed successfully. The surface roughness appeared to be similaramong the three groups. Ti-MgHA and Ti-Mg group had improved cellular responses with regard to the proliferation, alkaline phosphatase (ALP) activity, and bone-associated markers, such as bone sialoprotein (BSP) and osteocalcin (OCN) mRNA compared to those of Ti-S group. However, the differences between Ti-Mg group and Ti-MgHA group were not significant, in spite of the tendency of higher proliferation, ALP activity and BSP expression in Ti-MgHA group.
CONCLUSION
Mg and Mg-HAcoatings could stimulate the differentiation into osteoblastic MC3T3-E1 cells, potentially contributing to rapid osseointegration.

Keyword

Biocompatible materials; Surface-Coated materials; Surface properties; Alkaline phosphatase; Bone sialoprotein; Osteocalcin

MeSH Terms

Alkaline Phosphatase
Biocompatible Materials
Calcium Phosphates
Calcium*
Cell Adhesion
Cell Proliferation
Coated Materials, Biocompatible
Integrin-Binding Sialoprotein
Magnesium*
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Osseointegration
Osteoblasts*
Osteocalcin
Photoelectron Spectroscopy
Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger
Surface Properties
Titanium*
Alkaline Phosphatase
Biocompatible Materials
Calcium
Calcium Phosphates
Coated Materials, Biocompatible
Integrin-Binding Sialoprotein
Magnesium
Osteocalcin
RNA, Messenger
Titanium
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