J Korean Neurosurg Soc.  2015 Dec;58(6):508-512. 10.3340/jkns.2015.58.6.508.

Neuroprotective Effect of Resveratrol on Acute Brain Ischemia Reperfusion Injury by Measuring Annexin V, p53, Bcl-2 Levels in Rats

Affiliations
  • 1Department of Neurosurgery, Katip Celebi University Izmir Ataturk Training and Research Hospital, Izmir, Turkey.
  • 2Department of Neurosurgery, Eskisehir State Hospital, Eskisehir, Turkey.
  • 3Department of Pharmacology, Eskisehir Osmangazi University, Eskisehir, Turkey.
  • 4Department of Neurosurgery, Dokuz Eylul University, School of Medicine Hospital, Izmir, Turkey. okalemci@gmail.com
  • 5Department of Neurosurgery, Eskisehir Osmangazi University School of Medicine Hospital, Eskisehir, Turkey.

Abstract

BACKGROUND
Cerebral ischemia is as a result of insufficient cerebral blood flow for cerebral metabolic functions. Resveratrol is a natural phytoalexin that can be extracted from grape's skin and had potent role in treating the cerebral ischemia. Apoptosis, a genetically programmed cellular event which occurs after ischemia and leads to biochemical and morphological changes in cells. There are some useful markers for apoptosis like Bcl-2, bax, and p53. The last reports, researchers verify the apoptosis with early markers like Annexin V.
METHODS
We preferred in this experimental study a model of global cerebral infarction which was induced by bilateral common carotid artery occlusion method. Rats were randomly divided into 4 groups : sham, ischemia-reperfusion (I/R), I/R plus 20 mg/kg resveratrol and I/R plus 40 mg/kg resveratrol. Statistical analysis was performed using Sigmastat 3.5 ve IBM SPSS Statistics 20. We considered a result significant when p<0.001.
RESULTS
After administration of resveratrol, Bcl-2 and Annexin levels were significantly increased (p<0.001). Depending on the dose of resveratrol, Bcl2 levels increased, p53 levels decreased but Annexin V did not effected. P53 levels were significantly increased in ishemia group, so apoptosis is higher compared to other groups.
CONCLUSION
In the acute period, Annexin V levels misleading us because the apoptotic cell counts could not reach a certain level. Therefore we should support our results with bcl-2 and p53.

Keyword

Resveratrol; Brain Ischemia; Annexin V; Bcl-2; p53

MeSH Terms

Animals
Annexin A5*
Apoptosis
Brain Ischemia*
Brain*
Carotid Artery, Common
Cell Count
Cerebral Infarction
Ischemia
Neuroprotective Agents*
Rats*
Reperfusion Injury*
Reperfusion*
Skin
Annexin A5
Neuroprotective Agents
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