Immune Netw.  2010 Dec;10(6):212-218. 10.4110/in.2010.10.6.212.

The Stem Bark of Kalopanax pictus Exhibits Anti-inflammatory Effect through Heme Oxygenase-1 Induction and NF-kappaB Suppression

Affiliations
  • 1Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea. yheekim@pusan.ac.kr
  • 2Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.

Abstract

BACKGROUND
The stem bark of Kalopanax pictus (KP) has been used in traditional medicine to treat rheumatoidal arthritis, neurotic pain and diabetes mellitus in China and Korea. In this study, the mechanism responsible for anti-inflammatory effects of KP was investigated.
METHODS
We examined the effects of KP on NO production, nitric oxide synthase (iNOS) and HO-1 expression, NF-kappaB, Nrf2 and MAPK activation in mouse peritoneal macrophages.
RESULTS
The aqueous extract of KP inhibited LPS-induced NO secretion as well as inducible iNOS expression, without affecting cell viability. KP suppressed LPS-induced NF-kappaB activation, phosphorylation and degradation of IkappaB-alpha, phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). Furthermore, KP induced HO-1 expression and Nrf2 nuclear translocation.
CONCLUSION
These results suggest that KP has the inhibitory effects on LPS-induced NO production in macrophages through NF-kappaB suppression and HO-1 induction.

Keyword

Kalopanax pictus; NO; NF-kappaB; MAPK; HO-1; Nrf2

MeSH Terms

Animals
Arthritis
Cell Survival
China
Diabetes Mellitus
Heme
Heme Oxygenase-1
I-kappa B Proteins
JNK Mitogen-Activated Protein Kinases
Kalopanax
Korea
Macrophages
Medicine, Traditional
Mice
NF-kappa B
Nitric Oxide Synthase
Phosphorylation
Phosphotransferases
Heme
Heme Oxygenase-1
I-kappa B Proteins
JNK Mitogen-Activated Protein Kinases
NF-kappa B
Nitric Oxide Synthase
Phosphotransferases
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