Korean J Vet Res.  2015 Dec;55(4):233-240. 10.14405/kjvr.2015.55.4.233.

Anti-thrombotic activity of fermented rice bran extract with several oriental plants in vitro and in vivo

• Jeon BR
• Ji HD
• Kim SJ
• Lee CH
• Kim TW
• Rhee MH

• KMID: 2148066
• DOI: http://doi.org/10.14405/kjvr.2015.55.4.233

Abstract

Although the effects of the rice bran have recently been investigated, there is no information regarding platelet physiology available. However, it is well known that fermented natural plants have a beneficial effect on cardiovascular diseases. Therefore, this study was conducted to investigate whether fermented rice bran extract (FRBE) with several plants (Artemisia princeps, Angelica Gigantis Radix, Cnidium officinale, and Camellia sinensis) affected agonist-induced platelet aggregation, and if so, what the underlying mechanism of its activity was. We performed several experiments, including in vitro platelet aggregation, intracellular calcium concentration and adenosine triphosphate release. In addition, the activation of integrin alphaIIbbeta3 was determined using fibrinogen binding. Thrombus formation was also evaluated in vivo using an arterio-venous shunt model. The FRBE inhibited collagen-induced platelet aggregation in a concentration-dependent manner. FRBE significantly and dose dependently attenuated thrombus formation using rat arterio-venous shunt. FRBE suppressed the intracellular calcium mobilization in collagen-stimulated platelets. We also found that FRBE inhibited extracellular stimuli-responsive kinase 1/2, p38-mitogen-activated protein kinases and c-Jun N-terminal kinase phosphorylation. These results suggested that FRBE inhibited collagen-induced platelet aggregation, which was mediated by modulation of downstream signaling molecules. In conclusion, FRBE could be developed as a functional food against aberrant platelet activation-related cardiovascular diseases.

Keyword

collagen; mitogen-activated protein kinase; platelet aggregation; rice bran; thrombosis

MeSH Terms

Adenosine Triphosphate
Angelica
Animals
Blood Platelets
Calcium
Camellia
Cardiovascular Diseases
Cnidium
Collagen
Fibrinogen
Functional Food
JNK Mitogen-Activated Protein Kinases
Phosphorylation
Phosphotransferases
Physiology
Platelet Aggregation
Platelet Glycoprotein GPIIb-IIIa Complex
Protein Kinases
Rats
Thrombosis
Adenosine Triphosphate
Calcium
Collagen
Fibrinogen
JNK Mitogen-Activated Protein Kinases
Phosphotransferases
Platelet Glycoprotein GPIIb-IIIa Complex
Protein Kinases