J Korean Soc Plast Reconstr Surg.  2008 Nov;35(6):637-644.

The Effects of Human Adipose Tissue-derived Stem Cells on Degenerative Change of Knee in Rabbit Model

• Jeong KH
• Kim SK
• Jeong JO
• Heo J
• Kwon YS
• Lee KC

Abstract

PURPOSE: The survival of bone marrow derived stem cell was reported several times. But the survival of adipose tissue derived stem cells(hASCs) was not mentioned on. We studied the adipose tissue derived stem cell's survival and effect on articular cartilage in rabbits.
METHODS
Osteoarthritis was induced in twenty New Zealand white rabbits by intraarticular injection of monosodium iodoacetate(MIA). After four weeks, hASCs were also injected into the knee joints space without any vehicle, but the control group received phosphate buffered saline only. The histologic grade of articular cartilage was measured in 4 and 8 weeks after the transplantation of hASC and the viability of injected stem cells measured by Fluorescent in situ Hybridization (FISH) examination.
RESULTS
After 4 and 8 weeks from hASCs transplantation, histologic grade was not significantly difference between two groups(p>0.05), and the Y chromosome of the transplanted hASCs was not detected in articular cartilage.
CONCLUSION
We found that direct injection of hASC in joint space didn't work on damaged articular cartilage repair.

Keyword

hASCs; Stem cell; Osteoarthritis

MeSH Terms

Adipose Tissue
Bone Marrow
Cartilage, Articular
Humans
In Situ Hybridization, Fluorescence
Injections, Intra-Articular
Joints
Knee
Knee Joint
Osteoarthritis
Rabbits
Stem Cells
Transplants
Y Chromosome