J Korean Acad Rehabil Med.
2007 Jun;31(3):269-277.
The Effects of Human Adipose Tissue Derived Mesenchymal Stem Cells on Degenerative Change of Disc in Rabbit Model
- Affiliations
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- 1Department of Physical Medicine and Rehabilitation, Dong-A University College of Medicine, Korea. rm0007@hanafos.com
- 2Department of Plastic and Reconstructive Surgery, Dong-A University College of Medicine, Korea.
- 3Department of Laboratory Medicine, Dong-A University College of Medicine, Korea.
- 4Department of Pathology, Dong-A University College of Medicine, Korea.
Abstract
OBJECTIVE
To determine whether transplanted human adipose tissue derived stem cells (hATSCs) can survive and increase the amount of proteoglycans in degenerated intervertebral disc. METHOD: Lumbar disc degeneration was induced in thirty New Zealand white rabbits by injection of chondroitinase ABC(R). After 2 weeks, hATSCs were transplanted in degenerated disc in hATSCs group. Control group received phosphate buffered saline. The histologic grading and height of disc were measured at 2, 4, and 8 weeks after transplantation. The viability of donor cells was identified by using beta-Actin gene polymerase chain reaction (PCR).
RESULTS
4 and 8 weeks after hATSCs transplantation, the histologic grading showed significantly high score in hATSCs group (p<0.05), but the amount of proteoglycans was not significantly different between the two groups. The change of disc height was not significantly increased in hATSCs group. In the beta-Actin gene PCR analysis, positive signal in the hATSCs group was observed.
CONCLUSION
hATSCs transplantation may be useful in decelerating disc degeneration in experimental models and provide new hopes for treatment of degenerative disc disease in humans
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