Int J Stem Cells.  2015 Nov;8(2):121-127. 10.15283/ijsc.2015.8.2.121.

Adipose Tissue - Adequate, Accessible Regenerative Material

• Kolaparthy LK
• Sanivarapu S
• Moogla S
• Kutcham RS

• KMID: 2380806
• DOI: http://doi.org/10.15283/ijsc.2015.8.2.121

Abstract

The potential use of stem cell based therapies for the repair and regeneration of various tissues offers a paradigm shift that may provide alternative therapeutic solutions for a number of diseases. The use of either embryonic stem cells (ESCs) or induced pluripotent stem cells in clinical situations is limited due to cell regulations and to technical and ethical considerations involved in genetic manipulation of human ESCs, even though these cells are highly beneficial. Mesenchymal stem cells seen to be an ideal population of stem cells in particular, Adipose derived stem cells (ASCs) which can be obtained in large number and easily harvested from adipose tissue. It is ubiquitously available and has several advantages compared to other sources as easily accessible in large quantities with minimal invasive harvesting procedure, and isolation of adipose derived mesenchymal stem cells yield a high amount of stem cells which is essential for stem cell based therapies and tissue engineering. Recently, periodontal tissue regeneration using ASCs has been examined in some animal models. This method has potential in the regeneration of functional periodontal tissues because various secreted growth factors from ASCs might not only promote the regeneration of periodontal tissues but also encourage neovascularization of the damaged tissues. This review summarizes the sources, isolation and characteristics of adipose derived stem cells and its potential role in periodontal regeneration is discussed.

Keyword

Adipose tissue; Mesenchymal stem cells; Pluripotent stem cells; Periodontitis; Regeneration

MeSH Terms

Adipose Tissue*
Embryonic Stem Cells
Humans
Induced Pluripotent Stem Cells
Intercellular Signaling Peptides and Proteins
Mesenchymal Stromal Cells
Models, Animal
Periodontitis
Pluripotent Stem Cells
Regeneration
Social Control, Formal
Stem Cells
Tissue Engineering
Intercellular Signaling Peptides and Proteins

Reference

References

1. Mimeault M, Hauke R, Batra SK. Stem cells: a revolution in therapeutics-recent advances in stem cell biology and their therapeutic applications in regenerative medicine and cancer therapies. Clin Pharmacol Ther. 2007; 82:252–264. DOI: 10.1038/sj.clpt.6100301. PMID: 17671448.

2. Polak DJ. Regenerative medicine. Opportunities and challenges: a brief overview. J R Soc Interface. 2010; 7(Suppl 6):S777–S781. DOI: 10.1098/rsif.2010.0362.focus. PMID: 20826477. PMCID: 2988280.

3. Lenoir N. Europe confronts the embryonic stem cell research challenge. Science. 2000; 287:1425–1427. DOI: 10.1126/science.287.5457.1425. PMID: 10688780.

4. Ben-David U, Benvenisty N. The tumorigenicity of human embryonic and induced pluripotent stem cells. Nat Rev Cancer. 2011; 11:268–277. DOI: 10.1038/nrc3034. PMID: 21390058.

5. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284:143–147. DOI: 10.1126/science.284.5411.143. PMID: 10102814.

6. Fukuda K. Use of adult marrow mesenchymal stem cells for regeneration of cardiomyocytes. Bone Marrow Transplant. 2003; 32(Suppl 1):S25–S27. DOI: 10.1038/sj.bmt.1703940. PMID: 12931236.

7. Musina RA, Bekchanova ES, Sukhikh GT. Comparison of mesenchymal stem cells obtained from different human tissues. Bull Exp Biol Med. 2005; 139:504–509. DOI: 10.1007/s10517-005-0331-1. PMID: 16027890.

8. Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med. 2009; 4:265–273. DOI: 10.2217/17460751.4.2.265. PMID: 19317645.

9. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13:4279–4295. DOI: 10.1091/mbc.E02-02-0105. PMID: 12475952. PMCID: 138633.

10. Schipper B, Marra KG, Rubin JP. Regional anatomic and age effects on cell function of human adipose-derived stem cells. In : Fourth Annual International Fat Applied Technology Society; October 21–24, 2006; Baton Rouge, La. Abstract.

11. Van Harmelen V, Röhrig K, Hauner H. Comparison of proliferation and differentiation capacity of human adipocyte precursor cells from the omental and subcutaneous adipose tissue depot of obese subjects. Metabolism. 2004; 53:632–637. DOI: 10.1016/j.metabol.2003.11.012. PMID: 15131769.

12. Schipper BM, Marra KG, Zhang W, Donnenberg AD, Rubin JP. Regional anatomic and age effects on cell function of human adipose-derived stem cells. Ann Plast Surg. 2008; 60:538–544. DOI: 10.1097/SAP.0b013e3181723bbe. PMID: 18434829. PMCID: 4160894.

13. Prunet-Marcassus B, Cousin B, Caton D, André M, Pénicaud L, Casteilla L. From heterogeneity to plasticity in adipose tissues: site-specific differences. Exp Cell Res. 2006; 312:727–736. DOI: 10.1016/j.yexcr.2005.11.021. PMID: 16386732.

14. Rodbell M. Metabolism of isolated fat cells. II. The similar effects of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on glucose and amino acid metabolism. J Biol Chem. 1966; 241:130–139. PMID: 4379054.

15. Rodbell M, Jones AB. Metabolism of isolated fat cells. 3. The similar inhibitory action of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on lipolysis stimulated by lipolytic hormones and theophylline. J Biol Chem. 1966; 241:140–142. PMID: 4285132.

16. Kurita M, Matsumoto D, Shigeura T, Sato K, Gonda K, Harii K, Yoshimura K. Influences of centrifugation on cells and tissues in liposuction aspirates: optimized centrifugation for lipotransfer and cell isolation. Plast Reconstr Surg. 2008; 121:1033–1041. discussion 1042–1043. DOI: 10.1097/01.prs.0000299384.53131.87. PMID: 18317153.

17. Katz AJ, Llull R, Hedrick MH, Futrell JW. Emerging approaches to the tissue engineering of fat. Clin Plast Surg. 1999; 26:587–603. PMID: 10553215.

18. Boquest AC, Shahdadfar A, Brinchmann JE, Collas P. Isolation of stromal stem cells from human adipose tissue. Methods Mol Biol. 2006; 325:35–46. PMID: 16761717.

19. Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007; 100:1249–1260. DOI: 10.1161/01.RES.0000265074.83288.09. PMID: 17495232.

20. Mizuno H. Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review. J Nippon Med Sch. 2009; 76:56–66. DOI: 10.1272/jnms.76.56. PMID: 19443990.

21. Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008; 45:115–120. DOI: 10.1016/j.ymeth.2008.03.006. PMID: 18593609. PMCID: 3668445.

22. Huang JI, Zuk PA, Jones NF, Zhu M, Lorenz HP, Hedrick MH, Benhaim P. Chondrogenic potential of multipotential cells from human adipose tissue. Plast Reconstr Surg. 2004; 113:585–594. DOI: 10.1097/01.PRS.0000101063.27008.E1. PMID: 14758221.

23. Mizuno H, Zuk PA, Zhu M, Lorenz HP, Benhaim P, Hedrick MH. Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg. 2002; 109:199–209. DOI: 10.1097/00006534-200201000-00030. PMID: 11786812.

24. Lendeckel S, Jödicke A, Christophis P, Heidinger K, Wolff J, Fraser JK, Hedrick MH, Berthold L, Howaldt HP. Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report. J Craniomaxillofac Surg. 2004; 32:370–373. DOI: 10.1016/j.jcms.2004.06.002. PMID: 15555520.

25. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, Pell CL, Johnstone BH, Considine RV, March KL. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004; 109:1292–1298. DOI: 10.1161/01.CIR.0000121425.42966.F1. PMID: 14993122.

26. Collas P. Programming differentiation potential in mesenchymal stem cells. Epigenetics. 2010; 5:476–482. DOI: 10.4161/epi.5.6.12517. PMID: 20574163.

27. King JA, Miller WM. Bioreactor development for stem cell expansion and controlled differentiation. Curr Opin Chem Biol. 2007; 11:394–398. DOI: 10.1016/j.cbpa.2007.05.034. PMID: 17656148. PMCID: 2038982.

28. Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, Fraser JK, Hedrick MH. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005; 54:132–141. DOI: 10.2302/kjm.54.132. PMID: 16237275.

29. Tobita M, Mizuno H. Adipose derived stem cells and periodontal tissue engineering. Oral Craniofac Tissue Eng. 2011; 1:315–321.

30. Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008; 453:314–321. DOI: 10.1038/nature07039. PMID: 18480812.

31. Kilroy GE, Foster SJ, Wu X, Ruiz J, Sherwood S, Heifetz A, Ludlow JW, Stricker DM, Potiny S, Green P, Halvorsen YD, Cheatham B, Storms RW, Gimble JM. Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol. 2007; 212:702–709. DOI: 10.1002/jcp.21068. PMID: 17477371.

32. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, Pell CL, Johnstone BH, Considine RV, March KL. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004; 109:1292–1298. DOI: 10.1161/01.CIR.0000121425.42966.F1. PMID: 14993122.

33. Ebrahimian TG, Pouzoulet F, Squiban C, Buard V, André M, Cousin B, Gourmelon P, Benderitter M, Casteilla L, Tamarat R. Cell therapy based on adipose tissue-derived stromal cells promotes physiological and pathological wound healing. Arterioscler Thromb Vasc Biol. 2009; 29:503–510. DOI: 10.1161/ATVBAHA.108.178962. PMID: 19201690.

34. Cai L, Johnstone BH, Cook TG, Liang Z, Traktuev D, Cornetta K, Ingram DA, Rosen ED, March KL. Suppression of hepatocyte growth factor production impairs the ability of adipose-derived stem cells to promote ischemic tissue revascularization. Stem Cells. 2007; 25:3234–3243. DOI: 10.1634/stemcells.2007-0388. PMID: 17901400.

35. Tobita M, Mizuno H. Periodontal disease and periodontal tissue regeneration. Curr Stem Cell Res Ther. 2010; 5:168–174. DOI: 10.2174/157488810791268672.

36. Tobita M, Uysal AC, Ogawa R, Hyakusoku H, Mizuno H. Periodontal tissue regeneration with adipose-derived stem cells. Tissue Eng Part A. 2008; 14:945–953. DOI: 10.1089/ten.tea.2007.0048. PMID: 18558814.

37. Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004; 91:4–15.

38. Whitman DH, Berry RL, Green DM. Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg. 1997; 55:1294–1299. DOI: 10.1016/S0278-2391(97)90187-7. PMID: 9371122.