Korean J Phys Anthropol.  2014 Dec;27(4):235-243. 10.11637/kjpa.2014.27.4.235.

Carcinogenic Compounds, 2-nitrofluorene and Clonidine Can Modulate the Level of Sirtuin mRNAs Expression in Human Adipose Tissue-derived Stem Cells

• Lee J
• Lee CY
• Kwon Y
• Kim H
• Kim H

• KMID: 1982461
• DOI: http://doi.org/10.11637/kjpa.2014.27.4.235

Abstract

Sirtuins (SIRTs) are involved in multiple cellular processes. And they are involved in cellular pathways related aging, cancer, and a variety of cellular functions including cell cycle, DNA repair and proliferation. Also they modulate life span. Stem cells have the ability to self-renew for unlimited proliferation and differentiate into various cell types. It has been a little known that the mutation of undifferentiated stem cells in tissue may result in the development of cancer cells by genotoxic carcinogens. Therefore, this study investigated whether some carcinogenic compounds can modulate the expression of sirtuin mRNA on human adipose-derived stem cells. Adipose-derived stem cells (ADSC) were exposed to genotoxic carcinogenic compound (2-nitrofluorene, 2NF) and non-genotoxic carcinogenic compound (clonidine, CND) for 24 hours, 48 hours, 72 hours and 96 hours. The expression of SIRT1 mRNA increased on 72 hours. Expressions of SIRT2 and SIRT7 mRNA increased robustly on 48 hours. But all of SIRTs decreased to a level before a treatment of genotoxic compound on adipose-derived stem cells. These results demonstrated that a treatment of genotoxic compound induced the expression of SIRT mRNA only in the short time. But their level returned to untreated cells on 96 hours. They suggest that the possibility that the sirtuins can retard the carcinogenesis of adipose derived stem cells.

Keyword

Sirtuin; Human adipose derived stem cells; 2-nitrofluorene; Clonidine

MeSH Terms

Aging
Carcinogenesis
Carcinogens
Cell Cycle
Clonidine*
DNA Repair
Humans
RNA, Messenger*
Sirtuins
Stem Cells*
Carcinogens
Clonidine
RNA, Messenger
Sirtuins

Figure

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  Fig. 1. Morphology of cultured human adipose tissue-derived stem cells (hADSCs) is changed by treatment with carcinogenic compund, 2NF(78 μ M) and CND(600 μ M) for 24 hours, 48 hours, and 72 hours(×100).

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  Fig. 2. Growth rates of hADSC are decreased prominently by treat-ment with 2NF(78 μ M) for 24 hours, 48 hours, and 72 hours.

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  Fig. 3. Representative gel electrophoresis and analysis for determining sirtuin1 mRNA expression show increased levels in hADSC with treatment of carcinogenic compounds for 72 hours.

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  Fig. 4. Representative gel electrophoresis and analysis for determining sirtuin2 mRNA expression show increased levels in hADSC with treatment of 2NF and CND for 48 hours.

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  Fig. 5. Representative gel electrophoresis and analysis for determining sirtuin3 mRNA expression show increased levels in hADSC with treatment of 2NF and CND for 72 hours.

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  Fig. 6. Representative gel electrophoresis and analysis for determining sirtuin7 mRNA expression show increased levels in hADSC with treatment of 2NF and CND for 48 hours.

Reference

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