Korean J Phys Anthropol.  2014 Jun;27(2):91-99. 10.11637/kjpa.2014.27.2.91.

The Effects of Baicalein on Osteoclast Differentiation from Bone Marrow Derived Macrophage

• Yun JK
• Cheon YH
• Kim JY
• Kwak SC
• Yoon KH
• Baek JM
• Lee MS
• Oh J
• Park J

• KMID: 1917128
• DOI: http://doi.org/10.11637/kjpa.2014.27.2.91

Abstract

As prediction of rapidly aging society, bone health is considered increasingly important and received more attention than ever. Bone health is regulated by balancing between bone resorptive osteoclasts and bone formative osteoblasts. Disruption of balance between bone-resorbing osteoclasts and bone-forming osteoblasts results in bone disease. Natural products have recently received much attention as an alternative tool for the development of novel therapeutic strategy. Baicalein is reported it has anti-cancer, anti-inflammatory and antioxidant effects. Baicalein also has been known that it has both promotive effect on MC3T3-E1 cell line and inhibitory effect on RAW 264.7 cell line. However, the inhibitory mechanism of baicalein using bone marrow derived macrophages (BMMs) on osteoclast differentiation remains not clear. In this study, the suppressive mechanism by baicalein on osteoblast differentiation was evaluated. Bicalein inhibited receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation in BMMs in a dose dependent manner without any toxicity. Baicalein suppressed phosphorylation of protein kinaseB (Akt), c-Jun N-terminal kinases (JNK) and phosphoinositide-specific phospholipaseCgamma2 (PLCgamma2). Furthermore, Baicalein suppressed the induction of RANKL-induced c-Fos and Nuclear factor of activated T cell c1 (NFATc1), essential genes on osteoclastogenesis. In BMMs, Bicalein inhibited the mRNA expression of tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), cathepsinK, dendritic cell-specific transmembrane protein (DC-STAMP). Moreover, baicalein promoted differentiation of osteoblast on bone marrow stromal cells (BMSCs). Taken together, these results suggest that baicalein has a potential for treating bone lytic diseases, such as osteoporosis, periodontitis, and rheumatoid arthritis.

Keyword

Baicalein; Osteoclast; Osteoporosis; RANKL; PLCgamma2

MeSH Terms

Acid Phosphatase
Aging
Antioxidants
Arthritis, Rheumatoid
Biological Products
Bone Diseases
Bone Marrow*
Cell Line
Genes, Essential
Macrophages*
Mesenchymal Stromal Cells
Osteoblasts
Osteoclasts*
Osteoporosis
Periodontitis
Phospholipase C gamma
Phosphorylation
Phosphotransferases
RANK Ligand
RNA, Messenger
Acid Phosphatase
Antioxidants
Biological Products
Phospholipase C gamma
Phosphotransferases
RANK Ligand
RNA, Messenger