Korean J Parasitol.  2014 Apr;52(2):151-162.

Ameliorative Effect of Bone Marrow-Derived Stem Cells on Injured Liver of Mice Infected with Schistosoma mansoni

Affiliations
  • 1Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt. magda_elmahdi@hotmail.com
  • 2Department of Immunology, Theodor Bilharz Research Institute, Giza, Egypt.

Abstract

The technique of stem cells or hepatocytes transplantation has recently improved in order to bridge the time before whole-organ liver transplantation. In the present study, unfractionated bone marrow stem cells (BMSCs) were harvested from the tibial and femoral marrow compartments of male mice, which were cultured in Dulbecco's modified Eagle's medium (DMEM) with and without hepatocyte growth factor (HGF), and then transplanted into Schistosoma mansoni-infected female mice on their 8th week post-infection. Mice were sacrificed monthly until the third month of bone marrow transplantation, serum was collected, and albumin concentration, ALT, AST, and alkaline phosphatase (ALP) activities were assayed. On the other hand, immunohistopathological and immunohistochemical changes of granuloma size and number, collagen content, and cells expressing OV-6 were detected for identification of liver fibrosis. BMSCs were shown to differentiate into hepatocyte-like cells. Serum ALT, AST, and ALP were markedly reduced in the group of mice treated with BMSCs than in the untreated control group. Also, granuloma showed a marked decrease in size and number as compared to the BMSCs untreated group. Collagen content showed marked decrease after the third month of treatment with BMSCs. On the other hand, the expression of OV-6 increased detecting the presence of newly formed hepatocytes after BMSCs treatment. BMSCs with or without HGF infusion significantly enhanced hepatic regeneration in S. mansoni-induced fibrotic liver model and have pathologic and immunohistopathologic therapeutic effects. Also, this new therapeutic trend could generate new hepatocytes to improve the overall liver functions.

Keyword

Schistosoma mansoni; bone marrow; stem cell; liver; granuloma; collagen; OV-6; liver function

MeSH Terms

Alanine Transaminase/blood
Alkaline Phosphatase/blood
Animals
Antigens, Differentiation/biosynthesis
Aspartate Aminotransferases/blood
Bone Marrow Cells/cytology
*Bone Marrow Transplantation
Cell Differentiation
Cell- and Tissue-Based Therapy
Cells, Cultured
Collagen/metabolism
Female
Granuloma/parasitology/pathology
Hepatocyte Growth Factor/pharmacology
Hepatocytes/*cytology
Liver/parasitology/pathology
Liver Cirrhosis/parasitology/pathology/*therapy
Male
Mice
Mice, Inbred BALB C
Schistosoma mansoni/pathogenicity
Schistosomiasis mansoni/mortality/*therapy
*Stem Cell Transplantation
Stem Cells/cytology
Alanine Transaminase
Alkaline Phosphatase
Antigens, Differentiation
Aspartate Aminotransferases
Collagen
Hepatocyte Growth Factor
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