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Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts

Wirohadidjojo YW, Budiyanto , Soebono H

To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs...
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Isolation and characterization of a 40 kDa cysteine protease from Gymnophalloides seoi adult worms

Choi MH, Park WJ, Park YK, Chai JY, Lee SH

A 40 kDa cysteine protease was purified from the crude extract of adult worms of Gymnophalloides seoi by two consecutive steps: Sephacryl S-200 HR and DEAE-Sephacel chromatography. Enzyme activities were...
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Effects of Infrared Radiation on Skin Photo-Aging and Pigmentation

Lee JH, Roh MR, Lee KH

Infrared radiation is increasingly and uncritically used for cosmetic and wellness purposes, despite the poorly understood biologic effects of such treatments on humans. In the present study, we investigated the...
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Effect of Recombinant Human Epidermal Growth Factor Against Cutaneous Scar Formation in Murine Full-thickness Wound Healing

Kim YS, Lew DH, Tark KC, Rah DK, Hong JP

A visible cutaneous scar develops from the excess formation of immature collagen in response to an inflammatory reaction. This study examined the role of epidermal growth factor (EGF) in the...
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Characterization of UV-irradiated dense/porous collagen membranes: morphology, enzymatic degradation, and mechanical properties

Lee JE, Park JC, Hwang YS, Kim JK, Kim JG, Suh H

Collagen-based membranous materials of various shapes (gel, film, sponge) are known to be the most promising materials in terms of facilitating the regeneration of dermal defects. In...
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Purification of a 68-kDa cysteine proteinase from crude extract of Pneumocystis carinii

Choi MH, Chung BS, Chung YB, Yu JR, Cho SR, Hong ST

The present study intended to verify activities of cysteine proteinase of Pneumocystis carinii from rats and to purify the enzyme. In order to exclude the contamination of host-derived enzymes, concentrates...
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Differential Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Thioacetamide-Induced Chronic Liver Injury

Park SY, Shin HW, Lee KB, Lee MJ, Jang JJ

Hepatic fibrogenesis, a complex process that involves a marked accumulation of extracellular matrix components, activation of cells capable of producing matrix materials, cytokine release, and tissue remodeling, is regulated by...
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Partial characterization of a 29 kDa cysteine protease purified from Taenia solium metacestodes

Kim JY, Yang HJ, Kim KS, Chung YB

A 29 kDa cysteine protease of Taenia solium metacestodes was purified by Mono Q anion-exchanger and Superose 6 HR gel filtration chromatography. The enzyme was effectively inhibited by cysteine protease...
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Molecular Cloning and Characterization of a Paramyosin from Clonorchis sinensis

Park TJ, Kang JM, Na BK, Sohn WM

Paramyosin is a myofibrillar protein present in helminth parasites and plays multifunctional roles in host-parasite interactions. In this study, we identified the gene encoding paramyosin of Clonorchis sinensis (CsPmy) and...
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The Role of Focal Adhesion Kinase in the TGF-beta-Induced Myofibroblast Transdifferentiation of Human Tenon's Fibroblasts

Hong S, Lee JB, Iizuka Y, Song YK, Seong GJ, Han SH

PURPOSE: To investigate the role of focal adhesion kinase (FAK) in transforming growth factor (TGF)-beta-induced myofibroblast transdifferentiation of human Tenon's fibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to TGF-beta1...
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Collagenous Gastritis in A Korean Child: A Case Report

Park S, Kim DH, Choe YH, Suh YL

Collagenous gastritis, a counterpart of collagenous colitis, is an extremely rare disorder. The first case of collagenous gastritis in a Korean boy in his preteens who had been receiving treatment...
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Partial Purification and Properties of a Cysteine Protease from Citrus Red Mite Panonychus citri

Hong SC, Her KH, Kim HU, Lee J, Lee SP, Chung YB

Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify...
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Tempol Attenuates Renal Fibrosis in Mice with Unilateral Ureteral Obstruction: The Role of PI3K-Akt-FoxO3a Signaling

Yoon HE, Kim SJ, Kim SJ, Chung S, Shin SJ

This study investigated whether tempol, an anti-oxidant, protects against renal injury by modulating phosphatidylinositol 3-kinase (PI3K)-Akt-Forkhead homeobox O (FoxO) signaling. Mice received unilateral ureteral obstruction (UUO) surgery with or without...
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Overexpression of Transforming Growth Factor-beta1 in the Valvular Fibrosis of Chronic Rheumatic Heart Disease

Kim L, Kim DK, Yang WI, Shin DH, Jung IM, Park HK, Chang BC

For the purpose of determining the pathogenic role of transforming growth factor-beta1 (TGF-beta1) in the mechanism of chronic rheumatic heart disease, we evaluated the expression of TGF-beta1, proliferation of myofibroblasts,...
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Congenital Atrophoderma of Pasini and Pierini

Kim SK, Rhee SH, Kim YC, Lee ES, Kang HY

Idiopathic atrophoderma of Pasini and Pierini is a form of dermal atrophy of unknown etiology, usually affecting women during their adolescence and young adulthood. A 2-yr-old girl was presented with...
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Effect of intranasal rosiglitazone on airway inflammation and remodeling in a murine model of chronic asthma

Lee HY, Rhee CK, Kang JY, Park CK, Lee SY, Kwon SS, Kim YK, Yoon HK

BACKGROUND/AIMS: Asthma is characterized by airway hyperresponsiveness, inflammation, and remodeling. Peroxisome proliferator-activated receptors have been reported to regulate inflammatory responses in many cells. In this study, we examined the effects...
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Expression of alpha1 Receptor and Nitric Oxide Synthase in Oophorectomized and Estrogen-Supplemented Rat Bladder and Urethra

Seo Y, Park SW, Kim JY, Lee SD

PURPOSE: To investigate the effects of estrogen on the expression of the alpha1 receptor and nitric oxide synthase (NOS) in rat urethra and bladder after oophorectomy. MATERIALS AND METHODS: Forty-five mature...
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Angiogenesis and Mineralization During Distraction Osteogenesis

Choi IH, Chung CY, Cho TJ, Yoo WJ

Distraction osteogenesis is currently a standard method of bone lengthening. It is a viable method for the treatment of short extremities as well as extensive bone defects, because large amounts...
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Pulsed Electromagnetic Field Stimulates Cellular Proliferation in Human Intervertebral Disc Cells

Lee HM, Kwon UH, Kim H, Kim HJ, Kim B, Park JO, Moon ES, Moon SH

PURPOSE: The purpose of this study is to investigate the mechanism of cellular proliferation of electromagnetic field (EMF) on human intervertebral disc (IVD) cells. MATERIALS AND METHODS: Human IVD cells...
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Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs

Jee CH, Eom NY, Jang HM, Jung HW, Choi ES, Won JH, Hong IH, Kang BT, Jeong DW, Jung DI

This study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult...
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