Yonsei Med J.  2016 Sep;57(5):1282-1285. 10.3349/ymj.2016.57.5.1282.

Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts

• Wirohadidjojo YW
• Budiyanto
• Soebono H

• KMID: 2374177
• DOI: http://doi.org/10.3349/ymj.2016.57.5.1282

Abstract

To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm-2) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.

Keyword

Collagen; cell migration; platelet-rich fibrin; cell proliferation; ultraviolet-A

MeSH Terms

Blood Platelets/*cytology/*metabolism
Cell Movement/radiation effects
Cell Proliferation/radiation effects
Cells, Cultured
Collagen/metabolism
Fibrin/*metabolism
Fibroblasts/*cytology/metabolism/*radiation effects
Humans
Skin/*cytology
Time Factors
Ultraviolet Rays/*adverse effects
Collagen
Fibrin

Figure

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  Fig. 1 Migration of normal human dermal fibroblasts in Scratch-Assay. (A) Immediately after scratching. (B) 24 hours later. (C) 48 hours later. (D) 72 hours later.

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  Fig. 2 The effect of PRF-L on proliferation index, collagen deposition, and migration rates of UVA-irradiated HDFs. *p<0.05, †p<0.01. PRF-L, platelet-rich fibrin lysate; UVA, ultraviolet-A; HDFs, human dermal fibroblasts.

Reference

1. Harding KG, Moore K, Phillips TJ. Wound chronicity and fibroblast senescence--implications for treatment. Int Wound J. 2005; 2:364–368.

2. Clark RA. Oxidative stress and "senescent" fibroblasts in non-healing wounds as potential therapeutic targets. J Invest Dermatol. 2008; 128:2361–2364.

3. Margolis DJ, Morris LM, Papadopoulos M, Weinberg L, Filip JC, Lang SA, et al. Phase I study of H5.020CMV.PDGF-beta to treat venous leg ulcer disease. Mol Ther. 2009; 17:1822–1829.

4. Czuwara-Ladykowska J, Gore EA, Shegogue DA, Smith EA, Trojanowska M. Differential regulation of transforming growth factor-beta receptors type I and II by platelet-derived growth factor in human dermal fibroblasts. Br J Dermatol. 2001; 145:569–575.

5. Pan DB, Ke YS, Liu WJ, Wei YQ, Tang J, Cao H. [Platelet-derived growth factor-BB inhibited p21(WAF1) expression partially through transforming growth factor-beta signalling system in vascular smooth muscle cell]. Zhonghua Xin Xue Guan Bing Za Zhi. 2010; 38:160–165.

6. Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004; 91:4–15.

7. Ficarelli E, Bernuzzi G, Tognetti E, Bussolati O, Zucchi A, Adorni D, et al. Treatment of chronic venous leg ulcers by platelet gel. Dermatol Ther. 2008; 21:Suppl 1. S13–S17.

8. Dionyssiou D, Demiri E, Foroglou P, Cheva A, Saratzis N, Aivazidis C, et al. The effectiveness of intralesional injection of platelet-rich plasma in accelerating the healing of chronic ulcers: an experimental and clinical study. Int Wound J. 2013; 10:397–406.

9. Yin B, Jiang X. Telomere shortening in cultured human dermal fibroblasts is associated with acute photodamage induced by UVA irradiation. Postepy Dermatol Alergol. 2013; 30:13–18.

10. Naru E, Suzuki T, Moriyama M, Inomata K, Hayashi A, Arakane K, et al. Functional changes induced by chronic UVA irradiation to cultured human dermal fibroblasts. Br J Dermatol. 2005; 153:Suppl 2. 6–12.

11. Schwartz E, Cruickshank FA, Christensen CC, Perlish JS, Lebwohl M. Collagen alterations in chronically sun-damaged human skin. Photochem Photobiol. 1993; 58:841–844.

12. Chung JH, Seo JY, Choi HR, Lee MK, Youn CS, Rhie G, et al. Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol. 2001; 117:1218–1224.

13. Quan T, He T, Kang S, Voorhees JJ, Fisher GJ. Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol. 2004; 165:741–751.

14. Wlaschek M, Briviba K, Stricklin GP, Sies H, Scharffetter-Kochanek K. Singlet oxygen may mediate the ultraviolet A-induced synthesis of interstitial collagenase. J Invest Dermatol. 1995; 104:194–198.

15. Menter JM, Cornelison LM, Cannick L, Patta AM, Dowdy JC, Sayre RM, et al. Effect of UV on the susceptibility of acid-soluble Skh-1 hairless mouse collagen to collagenase. Photodermatol Photoimmunol Photomed. 2003; 19:28–34.

16. Dohan Ehrenfest DM, de Peppo GM, Doglioli P, Sammartino G. Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors. 2009; 27:63–69.

17. He L, Lin Y, Hu X, Zhang Y, Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108:707–713.

18. Taskiran D, Taskiran E, Yercan H, Kutay FZ. Quantification of total collagen in rabbit tendon by the sirius red method. Tr J Med Sci. 1999; 29:7–9.

19. Yarrow JC, Perlman ZE, Westwood NJ, Mitchison TJ. A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods. BMC Biotechnol. 2004; 4:21.

20. Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg. 2004; 114:1502–1508.

21. Kim MS, Song HJ, Lee SH, Lee CK. Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts. J Cosmet Dermatol. 2014; 13:44–51.

22. Porsch H, Mehic´ M, Olofsson B, Heldin P, Heldin CH. Platelet-derived growth factor β-receptor, transforming growth factor β type I receptor, and CD44 protein modulate each other's signaling and stability. J Biol Chem. 2014; 289:19747–19757.

23. Villeneuve J, Block A, Le Bousse-Kerdilès MC, Lepreux S, Nurden P, Ripoche J, et al. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Exp Hematol. 2009; 37:849–856.

24. Lacarrubba F, Tedeschi A, Nardone B, Micali G. Mesotherapy for skin rejuvenation: assessment of the subepidermal low-echogenic band by ultrasound evaluation with cross-sectional B-mode scanning. Dermatol Ther. 2008; 21:Suppl 3. S1–S5.

25. Suswardana , Radiono S, Chusniyati N, Rosmelia , Wirohadidjojo YW. The effect of 50% glycolic acid on the percutaneous absorption of eutectic mixture of local anesthetics (EMLA): a study of the electrofulguration-induced pain. Int J Dermatol. 2008; 47:280–283.