Korean J Parasitol.  2002 Mar;40(1):55-58. 10.3347/kjp.2002.40.1.55.

Effects of exogenous glucose on survival and infectivity of Schistosoma mansoni cercariae

• Fried B
• Laterra R
• Kim YH

• KMID: 1384712
• DOI: http://doi.org/10.3347/kjp.2002.40.1.55

Abstract

The effects of exogenous glucose in artificial spring water (ASW) were studied on the survival and infectivity of Schistosoma mansoni cercariae. The mean percent survival of cercariae maintained in 1 % glucose in ASW for 36 and 48 hr was significantly greater than that of cercariae maintained identically in ASW. Cercariae maintained in ASW with or without glucose for 24 hr, fixed in neutral buffered formalin, and stained in Oil Red O, showed an accumulation of neutral lipid in the tail. Cercariae maintained as described above and stained in periodic acid-Schiff exhibited depleted glycogen, mainly from the tail. Cercariae maintained in ASW with glucose for 24 hr did not resynthesize glycogen. Cercariae maintained in ASW with glucose for 24 hr were as capable of infecting male FVBN202 mice as were freshly emerged cercariae, and increased the percent of worm recovery. Exogeneous glucose added to ASW prolonged the survival of S. mansoni cercariae and increased infectivity in terms of worm recovery.

Keyword

Schistosoma mansoni; trematodes; cercariae; glucose; survival; infectivity

MeSH Terms

Animals
Glucose/*pharmacology
Larva
Male
Mice
Mice, Inbred Strains
Schistosoma mansoni/*growth & development/*pathogenicity
Stimulation, Chemical
Time Factors
Water