J Vet Sci.
2014 Mar;15(1):27-33. 10.4142/jvs.2014.15.1.27.
Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
- Affiliations
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- 1Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea. ysyoon@snu.ac.kr
- 2Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, Korea.
- 3Department of Anatomy and Physiology, College of Pharmacy, Dankook University, Cheonan 330-714, Korea.
- 4Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Korea.
- 5Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea.
- KMID: 1737607
- DOI: http://doi.org/10.4142/jvs.2014.15.1.27
Abstract
- In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.
MeSH Terms
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Animals
Blotting, Western
Brain-Derived Neurotrophic Factor/metabolism
Cell Differentiation/*drug effects
Cell Proliferation/drug effects
Dentate Gyrus/growth & development/physiology
Diet, Fat-Restricted
*Diet, High-Fat
Hippocampus/growth & development/physiology
Hypoglycemic Agents/*pharmacology
Immunohistochemistry
Ki-67 Antigen/metabolism
Male
Mice, Inbred C57BL
Microtubule-Associated Proteins/metabolism
Neurogenesis/*drug effects
Neuropeptides/metabolism
Thiazolidinediones/*pharmacology
Brain-Derived Neurotrophic Factor
Hypoglycemic Agents
Ki-67 Antigen
Microtubule-Associated Proteins
Neuropeptides
Thiazolidinediones
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