J Korean Neurosurg Soc.  1992 Jul;21(7):813-825.

A Study on the Change of Xanthine Oxidase Activities in Experimental Cerebral Infarction

• Min BK
• Park K
• Kim YB
• Hwang SN
• Suk JS
• Choi DY
• Park YS
• Lee YB

Abstract

To observe the activity of the xanthine oxidase in cerebral ischemia, an ischemic model was made using a transorbital occlusion of the middle cerebral artery(MCA) in cats. Xanthine oxidase activity was measured by means of high performance liquid chromatography with electrochemical detection of uric acid. The results were as follows: The infarction in the territory of the left middle cerebral artery(MCA) was identified with intracardiac perfusion of a 2% triphenyltetrazolium chloride(TTC) solution after transorbital occusion. 2) There was no significant difference between the control and ischemia groups in the total xanthine oxidase activity. 3) In the control group, 38.2% of the total xanthine oxidase was in the oxygen-dependent superoxide-producing oxidase group(Type 0). It had been increased with time in ischemia, despite the total xanthine oxidase activity remaining the same;particularly at 12 hours(p<0.05) and 24 hours(p<0.01) after occlusion of the left MCA. 4) In the healthy side, the activity of type 0 and its ratio to the total xanthine oxidase activity has not been increased. 5) In the ischemic side, the conversion of type D xanthine oxidase to the type 0 xanthine oxidase was demonstrated and its rate had been increased with the passage of time.

Keyword

Cerebral ischemia; Free radicals; High-performance liquid chromatography; Xanthine oxidase

MeSH Terms

Animals
Brain Ischemia
Cats
Cerebral Infarction*
Chromatography, Liquid
Free Radicals
Infarction
Ischemia
Oxidoreductases
Perfusion
Uric Acid
Xanthine Oxidase*
Xanthine*
Free Radicals
Oxidoreductases
Uric Acid
Xanthine
Xanthine Oxidase