Korean J Gastroenterol.  2001 Sep;38(3):161-168.

Alimenary Tract: Expression of Cyclooxygenase - 2 and Inducible Nitric Oxide Synthase mRNA in Gastric Cancer

• Park DI
• Rhee JC
• Son HJ
• Song SY
• Kim YH
• Rhee PL
• Kim JJ
• Paik SW
• Choi KW
• Son TS

Abstract

BACKGROUND/AIMS: It has been expected that chronic expression of cyclooxygenase-2(COX-2) and inducible nitric oxide synthase(Inos) may play an important role in gastric carcinogenesis. The purpose of our study was to investigate the role of COX-2 and Inos Mrna expression in gastric carcinogenesis and the correlation between the expression of COX-2 and Inos Mrna in patients with gastric cancer.
METHODS
Twenty-three gastric carcinoma tissue specimens and their adjacent normal mucosa specimens were obtained from surgical resection. The expression of COX-2 and Inos Mrna were examined by comparative reverse transcriptase-polymerase chain reaction.
RESULTS
COX-2 and Inos Mrna were expressed significantly higher in gastric cancer tissues than in adjacent normal tissues. There was a significant correlation between the level of COX-2 and Inos Mrna in carcinoma tissues. There was no significant correlation between the level of COX-2 or Inos Mrna expression and clinicopathological parameters such as tumor size, depth of invasion, differentiation, Laurens classification, lymph node metastasis, perineural and vascular invasion in these patients.
CONCLUSIONS
The expression of COX-2 and Inos may be one of the factors that contribute to the early stage of gastric carcinogenesis.

Keyword

Cyclooxygenase-2; Inducible nitric oxide synthase; Gastric cancer

MeSH Terms

Carcinogenesis
Classification
Cyclooxygenase 2
Humans
Lymph Nodes
Mucous Membrane
Neoplasm Metastasis
Nitric Oxide
Nitric Oxide Synthase Type II*
Prostaglandin-Endoperoxide Synthases*
RNA, Messenger*
Stomach Neoplasms*
Cyclooxygenase 2
Nitric Oxide
Nitric Oxide Synthase Type II
Prostaglandin-Endoperoxide Synthases
RNA, Messenger