Korean J Anat.  2009 Sep;42(3):179-185.

Effect of Water Extract of Papaya on RANKL-induced Osteoclast Differentiation

• Kim KM
• Lee CH
• Kim YK
• Oh J
• Kwak HB
• Kim JJ

Abstract

Balance between bone-resorbing osteoclats and bone-forming osteoblasts is important in bone homeostasis. In particular, increased osteoclast formation and activity are responsible for bone diseases such as osteoporosis, rheumatoid arthritis, periodontal disease. Natural metabolites of plants have recently received much attention as lead compounds for the development of novel therapeutic strategy. The purpose of this study was to search the natural products to inhibition osteoclast differentiation. Water extract of papaya significantly inhibited receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation in bone marrow macrophages (BMMs) in a dose dependent manner. However, water extract of papaya did not affect cytotoxicity when compared with control. Water extract of papaya inhibited the phosphorylation of p38 and JNK induced by RANKL. The mRNA expression of c-Fos, NFATcl, TRAP and OSCAR induced by RANKL was inhibited by water extract of papaya treatment. Also, water extract of papaya suppressed the protein expression of c-Fos and NFATc1 in BMMs treated with RANKL. Taken together, these results suggest that papaya may be a useful drug in the treatment of bone-related disease.

Keyword

Bone; Papaya; Osteoclast; RANKL

MeSH Terms

Arthritis, Rheumatoid
Biological Agents
Bone Diseases
Bone Marrow
Carica
Homeostasis
Macrophages
Osteoblasts
Osteoclasts
Osteoporosis
Periodontal Diseases
Phosphorylation
RANK Ligand
RNA, Messenger
Water
Biological Agents
RANK Ligand
RNA, Messenger
Water