Korean J Intern Med.
2019 Nov;34(6):1279-1286. 10.3904/kjim.2018.145.
Effect of lutein on methotrexate-induced oxidative lung damage in rats: a biochemical and histopathological assessment
- Affiliations
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- 1Department of Pharmacology, Erzincan University Faculty of Medicine, Erzincan, Turkey. renad_mamedov@hotmail.com
- 2Department of Family Medicine, Erzincan University Faculty of Medicine, Erzincan, Turkey.
- 3Department of Pathology, Erzincan University Faculty of Medicine, Erzincan, Turkey.
- 4Department of Biochemistry, Ataturk University Faculty of Medicine, Erzurum, Turkey.
- 5Department of Nursing, Erzincan University Faculty of Health Sciences, Erzincan, Turkey.
- 6Department of Anatomy, Ataturk University Faculty of Medicine, Erzurum, Turkey.
- KMID: 2463486
- DOI: http://doi.org/10.3904/kjim.2018.145
Abstract
- BACKGROUND/AIMS
This study aimed to investigate the effect of lutein on methotrexate (MTX)-induced pulmonary toxicity in rats biochemically and histopathologically.
METHODS
The rats in the MTX + lutein (MTXL, n = 6) group were given 1 mg/kg of lutein orally. A 0.9% NaCl solution was administered orally to the MTX (n = 6) group and the healthy group (HG, n = 6). One hour later, a single 20 mg/kg dose of MTX was injected intraperitoneally in the MTXL and MTX. Lutein or 0.9% NaCl solution was administered once a day for 5 days. At the end of this period, malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), interleukin 1 beta (IL-1β), and tumor necrosis factor alpha (TNF-α) were measured in the lung tissues from the animals euthanized with 50 mg/kg thiopental sodium anesthesia. Subsequently, histopathological examinations were performed.
RESULTS
The levels of MDA, MPO, IL-1β, and TNF-α in the lung tissue of the MTX were significantly higher than those of the MTXL and HG groups (p < 0.0001), and the amount of tGSH was lower. The histopathological findings in the MTX group, in which the oxidants and cytokines were higher, were more severe.
CONCLUSIONS
Lutein prevented the MTX-induced oxidative lung damage biochemically and histopathologically. This result indicates that lutein may be useful in the treatment of MTX-induced lung damage.
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