J Periodontal Implant Sci.  2018 Dec;48(6):383-394. 10.5051/jpis.2018.48.6.383.

Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model

Affiliations
  • 1Department of Periodontology, Gangneung-Wonju National University College of Dentistry, Gangneung, Korea. periojk@gwnu.ac.kr
  • 2Department of Anatomy, Gangneung-Wonju National University College of Dentistry, Gangneung, Korea.
  • 3Research Institute for Dental Engineering, Gangneung-Wonju National University, Gangneung, Korea. cwy@gwnu.ac.kr
  • 4Department of Metal and Materials Engineering, Gangneung-Wonju National University, Gangneung, Korea.
  • 5Wellnanos Co., Ltd., Gangneung, Korea.

Abstract

PURPOSE
The purpose of this study was to evaluate the optimal diabetes duration for bone regeneration experiments in an alloxan monohydrate (ALX)-induced diabetic rabbit calvarial defect model by evaluating the association between diabetes duration and bone healing capacity.
METHODS
Twenty-four New Zealand white rabbits were used. Twenty-two rabbits were injected with 100 mg/kg of ALX to induce experimental diabetes. These rabbits were divided into 4 groups, including a control group and groups with diabetes durations of 1 week (group 1), 2 weeks (group 2), and 4 weeks (group 3). Calvarial defects were created at 1, 2, and 4 weeks after ALX injection and in the control rabbits. Cone-beam computed tomography (CBCT) scanning was performed on the day of surgery and at 2 and 4 weeks after surgery. The rabbits were sacrificed 4 weeks after surgery, followed by histological and immunofluorescence analysis.
RESULTS
The diabetic state of all diabetic rabbits was well-maintained throughout the experiment. Reconstructed 3-dimensional CBCT imaging showed more rapid and prominent bone regeneration in the control group than in the experimental groups. Histological staining showed notable bone regeneration in the control group, in contrast to scarce bone formation in the experimental groups. The appearance and immunoreactivity of receptor activator of nuclear factor-kappa B and osteoprotegerin did not show notable differences among the groups.
CONCLUSION
ALX administration at 100 mg/kg successfully induced experimental diabetes in rabbits. The effect of diabetes on bone healing was evident when the interval between diabetes induction and the intervention was ≥1 week.

Keyword

Alloxan; Bone regeneration; Cone-beam computed tomography; Experimental diabetes mellitus; Rabbits

MeSH Terms

Alloxan
Animals
Bone Regeneration*
Cone-Beam Computed Tomography
Diabetes Mellitus, Experimental
Fluorescent Antibody Technique
Osteogenesis
Osteoprotegerin
Rabbits
Receptor Activator of Nuclear Factor-kappa B
Alloxan
Osteoprotegerin
Receptor Activator of Nuclear Factor-kappa B
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