J Korean Assoc Maxillofac Plast Reconstr Surg.  2012 Sep;34(5):293-298.

The Effects of Tetracycline-loaded Silk Fibroin Membrane on Guided Bone Regeneration in a Rabbit Calvarial Defect Model

• Lee SW
• Park YT
• Kim SG
• Kweon HY
• Jo YY
• Lee HS

Abstract

PURPOSE
The aim of this study was to evaluate the bone regeneration ability of 1% tetracycline (TC)-loaded silk fibroin membrane (SFM), in a rabbit calvarial defect model.
METHODS
Twenty New Zealand white rabbits were used for this study. Bilateral round defects were made on the rabbit parietal bone, using trephine bur with an 8 mm diameter. TC-loaded SFM or SFM was covered on the right parietal bone defect, and the left parietal bone defects were uncovered for the control. The animals were humanely sacrificed at 4 or 8 weeks postoperatively. A micro-computerized tomography (micro-CT) of each specimen was taken for analysis of bone regeneration. Hematoxylin and Eosin stain were done to observe histological findings.
RESULTS
From the micro-CT results, regenerated bone volume (mm3) of 1% TC-loaded SFM, SFM, and control were 7.80+/-5.87, 8.79+/-3.44, and 10.61+/-5.3 at 4 weeks postoperatively, respectively (P>0.05). Regenerated bone volume (mm3) of 1% TC-loaded SFM, SFM, and control were 36.56+/-8.50, 25.86+/-8.17, and 19.09+/-5.07 at 8 weeks postoperatively, respectively (P<0.05).
CONCLUSION
The 1% TC-loaded SFM showed more bone regeneration than the SFM and the uncovered control, in guided bone regeneration.

Keyword

Tetracycline; Silk fibroin; Guided bone regeneration

MeSH Terms

Animals
Bone Regeneration
Eosine Yellowish-(YS)
Fibroins
Hematoxylin
Humans
Membranes
Parietal Bone
Rabbits
Silk
Tetracycline
Eosine Yellowish-(YS)
Fibroins
Hematoxylin
Silk
Tetracycline