J Korean Assoc Oral Maxillofac Surg.  2010 Aug;36(4):250-254. 10.5125/jkaoms.2010.36.4.250.

The bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) in the calvaria defect of rabbit

  • 1Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea. epken@chol.com
  • 2National Academy of Agricultural Science, RDA, Suwon, Korea.


This study evaluated the bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) of a bone defect in rabbits.
Ten New Zealand white rabbits were used for this study and bilateral round shaped defects were formed in the parietal bone (diameter: 8.0 mm). The silk fibroin mixed with PRF was grafted into the right parietal bone (experimental group). The left side (control group) was grafted only PRF. The animals were sacrificed at 4 weeks and 8 weeks. A micro-computerized tomography (microCT) of each specimen was taken. Subsequently, the specimens were decalcified and stained for histological analysis.
The average value of plane film analysis was higher in the experimental group than in the control group at 4 weeks and 8weeks after surgery. However, the difference was not statistically significant.(P>0.05) The tissue mineral density (TMD) in the experimental group at 4 weeks after surgery was significantly higher than the control group.(P<0.05)
Silk fibroin can be used as a scaffold of PRF for rabbit calvarial defect repair.


Platelet-rich fibrin (PRF); Silk fibroin; Bone regeneration

MeSH Terms

Bone Regeneration
Parietal Bone


  • Fig. 1. After centrifugation, the blood was separated in 3 layer. We used middle layer for grafting.

  • Fig. 2. The silk protein mixed with PRF was grafted into the right parietal bone (experimental group: asterisk) and the left side (control group) was grafted only PRF. (PRF: platelet-rich fibrin)

  • Fig. 3. Percent radiopacities within the PRF (test) and PRF+Silk (control) defects.

  • Fig. 4. Histologic section (H&E staining, original magnification ×100). Osteoid formation both in the experimental group and in the control group at 4 weeks and 8 weeks.

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