Asian Spine J.
2018 Oct;12(5):785-793. 10.31616/asj.2018.12.5.785.
Sensory and Motor Behavior Evidences Supporting the Usefulness of Conditioned Medium from Dental Pulp-Derived Stem Cells in Spinal Cord Injury in Rats
- Affiliations
-
- 1Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. dehghanf@sums.ac.ir
- 2Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
- 3Cellular and Molecular Medicine Student Research Group, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran.
- 4Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
- 5Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
- 6Department of Neurosurgery, Shiraz University of Medical Sciences, Shiraz, Iran.
- 7Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
- 8Recombinant Protein Laboratory, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
- KMID: 2426637
- DOI: http://doi.org/10.31616/asj.2018.12.5.785
Abstract
- STUDY DESIGN: Experimental animal study.
PURPOSE: This study aimed to assess effects of conditioned medium (CM) of dental pulp-derived stem cells loaded in collagen hydrogel on functional recovery following spinal cord injury (SCI).
OVERVIEW OF LITERATURE: SCI affects sensory and motor functions, and behavioral recovery is the most essential purpose of therapeutic intervention. Recent studies have reported that CM from dental pulp-derived stem cells has therapeutic benefits. In addition, collagen hydrogel acts as a drug delivery system in SCI experiments.
METHODS
Stem cells from human exfoliated deciduous teeth (SHEDs) were cultured, and SHED-CM was harvested and concentrated. Collagen hydrogel containing SHED-CM was prepared. The rats were divided into five groups receiving laminectomy, compressive SCI with or without intraspinal injection of biomaterials (SHED-CM), and collagen hydrogel with or without SHED-CM. Basso, Beattie, and Bresnahan (BBB) scoring, inclined plane, cold allodynia, and beam walk tests were performed for 6 weeks to assess locomotor, motor, sensory, and sensory-motor performances, respectively.
RESULTS
Scores of the rats receiving SHED-CM loaded in collagen hydrogel were significantly better than those of the other injured groups at 1-week post-injury for BBB, 2 weeks for inclined plane, 2 weeks for cold allodynia, and 4 weeks for beam walk tests (p < 0.05). The differences remained significant throughout the study.
CONCLUSIONS
Intraspinal administration of SHED-CM loaded in collagen hydrogel leads to improved functional recovery and proposes a cell-free therapeutic approach for SCI.
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