Int J Stem Cells.  2020 Jul;13(2):257-267. 10.15283/ijsc20027.

Effect of Conditioned Medium from Human Umbilical Cord-Derived Mesenchymal Stromal Cells on Rejuvenation of Nucleus Pulposus Derived Stem/Progenitor Cells from Degenerated Intervertebral Disc

Affiliations
  • 1Department of Biochemistry and Molecular Biology, School of Preclinical Medicine, Jinan University, Guangzhou, China
  • 2Department of Orthopedic Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, China
  • 3Department of Materials Science and Engineering, Jinan University, Guangzhou, China
  • 4Department of Orthopedic Surgery, Centro Hospitalar Conde de Sao Januario, Macao, China

Abstract

Background and Objectives
Mesenchymal stromal cells (MSCs)-based treatment for degeneration of intervertebral disc (IVD) has been proposed recently. We here addressed whether MSC secreted factors can rejuvenate nucleus pulposus- derived stem/progenitor cells from degenerated disc (D-NPSCs) in vitro.
Methods and Results
We analyzed the expression of MSCs and NP cell specific surface markers, pluripotency related genes, multilineage potential and cell proliferative capacity of D-NPSCs upon incubation with the conditioned medium which was collected from the umbilical cord derived MSCs (UCMSCs). Our results indicated that the conditioned medium restore the stemness of D-NPSCs by up-regulating the expression level of CD29 and CD105, pluripotency related genes OCT4 and Nanog, and NP progenitor marker Tie2. The increased stemness was accompanied by promoted cell proliferative capacity and improved osteogenic and chondrogenic differentiation potential.
Conclusions
Our findings suggested that the UCMSCs derived conditioned medium might be used to rejuvenate the degenerated NP stem/progenitor cells.

Keyword

Conditioned medium; Mesenchymal stromal cells; Nucleus pulposus derived stem/progenitor cells; Intervertebral disc degeneration
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