Immune Netw.  2002 Sep;2(3):166-174. 10.4110/in.2002.2.3.166.

Differentiation of Osteoblast Progenitor Cells from Human Umbilical Cord Blood

Affiliations
  • 1Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea, 3Histostem Corporation, Seoul, Korea. cbib@catholic.ac.kr
  • 2Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • 3Histostem Corporation, Seoul, Korea.

Abstract

BACKGROUND: Human umbilical cord bloods, which could be taken during the delivery are utilized as a source of hematopoietic stem cells. Also in cord blood, there are several kinds of stem cells such as endothelial and mesenchymal stem cells.
METHODS
We isolated the mesenchymal stem cells from human umbilical cord bloods and confirmed the differentiation of these cells into osteoblast progenitor cells. The mesenchymal stem cells derived from umbilical cord blood have the ability to differentiate into specific tissue cells, which is one of characteristics of stem cells. These cells were originated from the multipolar shaped cells out of adherent cells of the umbilical cord blood mononuclear cell culture.
RESULTS
The mesenchymal stem cells expressed cell surface antigen CD13, CD90, CD102, CD105, alpha-smooth muscle actin and cytoplasmic antigen vimentine. Having cultrued these cells in bone formation media, we observed the formation of extracellular matrix and the expression of alkaline phosphatase and of mRNA of cbfa-1, ostoecalcin and type I collagen.
CONCLUSION
From these results we concluded that the cells isolated from the umbilical cord blood were mesenchymal stem cells, which we could differentiate into osteoblast when cultured in bone formation media. In short, it is suggested that these cells could be used as a new source of stem cells, which has the probability to alternate the embryonic stem cells.

Keyword

Human umbilical cord blood; mesenchymal stem cells; osteoblast progenitor cell

MeSH Terms

Actins
Alkaline Phosphatase
Antigens, Surface
Cell Culture Techniques
Collagen Type I
Cytoplasm
Embryonic Stem Cells
Extracellular Matrix
Fetal Blood*
Hematopoietic Stem Cells
Humans*
Mesenchymal Stromal Cells
Osteoblasts*
Osteogenesis
RNA, Messenger
Stem Cells*
Umbilical Cord*
Vimentin
Actins
Alkaline Phosphatase
Antigens, Surface
Collagen Type I
RNA, Messenger
Vimentin
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