J Korean Assoc Oral Maxillofac Surg.  2005 Feb;31(1):39-45.

Endothelial progenitor cells and mesenchymal stem cells from human cord blood

Affiliations
  • 1Department of Dentistry, College of Medicine, Chungnam National University, Korea. eskim@cnu.ac.kr
  • 2Department of Diagnosis and Laboratory Medicine, College of Medicine, Yonsei University, Korea.

Abstract

Stem cell therapy using mesenchymal stem cells(MSCs) transplantation have been paid attention because of their powerful proliferation and pluripotent differentiating ability. Although umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, the presence of mesenchymal stem cells (MSCs) in UCB has been controversial and it remains to be validated. In this study, we examine the presence of MSCs in UCB harvests and the prevalence of them is compared to that of endothelial progenitor cells. For this, CD34+ and CD34- cells were isolated and cultured under the endothelial cell growth medium and mesenchymal stem cell growth medium respectively. The present study showed that ESC-like cells could be isolated and expanded from preterm UCBs but were not acquired efficiently from full-terms. They expressed CD14-, CD34-, CD45-, CD29+, CD44+, CD105+ cell surface marker and could differentiate into adipogenic and osteogenic lineages. Our results suggest that MSCs are fewer in full-term UCB compared to endothelial progenitor cells.

Keyword

Mesenchymal stem cell; Endothelial progenitor cell; Umbilical cord; Blood

MeSH Terms

Endothelial Cells
Fetal Blood*
Hematopoietic Stem Cells
Humans*
Mesenchymal Stromal Cells*
Prevalence
Stem Cells*
Umbilical Cord
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