Clin Orthop Surg.  2017 Jun;9(2):239-248. 10.4055/cios.2017.9.2.239.

Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties

• Kim EJ
• Seo SG
• Shin HS
• Lee DJ
• Kim JH
• Lee DY

• KMID: 2412295
• DOI: http://doi.org/10.4055/cios.2017.9.2.239

Abstract

BACKGROUND
The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investigated their potential as a reliable source of stem cells for cell-based therapy.
METHODS
De-identified subcutaneous fat tissues were harvested after amputation in CLI patients. Freshly isolated SVF cells and culture-expanded adipose-derived stem cells (ADSCs) were quantified using flow cytometry. A matrigel tube formation assay and multi-lineage differentiation were performed to assess pericytic and mesenchymal stem cell (MSC)-like characteristics of PDGFRβ+ ADSCs.
RESULTS
PDGFRβ+ cells were located in the pericytic area of various sizes of blood vessels and coexpressed mesenchymal stem cell markers. PDGFRβ+ cells in freshly isolated SVF cells expressed a higher level of stem cell markers (CD34 and CXCR4) and mesenchymal markers (CD13, CD44, CD54, and CD90) than PDGFRβ- cells. In vitro expansion of PDGFRβ+ cells resulted in enrichment of the perivascular mesenchymal stem-like (PDGFRβ+/CD90+/CD45-/CD31-) cell fractions. The Matrigel tube formation assay revealed that PDGFRβ+ cells were located in the peritubular area.
CONCLUSIONS
PDGFRβ+ ADSCs cells demonstrated a good multilineage differentiation potential. Pericyte-like PDGFRβ+ cells from the SVF of adipose tissue from CLI patients had MSC-like characteristics and could be amplified by in vitro culture with preservation of their cell characteristics. We believe PDGFRβ+ cells in the SVF of adipose tissue can be used as a reliable source of stem cells even in CLI patients.

Keyword

Platelet-derived growth factor; Adipose tissue; Adult stem cells; Ischemia

MeSH Terms

Adipose Tissue, White/*cytology/metabolism
Amputation
Cells, Cultured
Humans
Ischemia/*metabolism
Mesenchymal Stromal Cells/cytology/*metabolism
Pericytes/cytology/*metabolism
Platelet-Derived Growth Factor/*metabolism
Receptors, Platelet-Derived Growth Factor/*metabolism
Platelet-Derived Growth Factor
Receptors, Platelet-Derived Growth Factor

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