Ann Coloproctol.  2017 Aug;33(4):139-145. 10.3393/ac.2017.33.4.139.

Anaphase-Promoting Complex 7 is a Prognostic Factor in Human Colorectal Cancer

• Kim IY
• Kwon HY
• Park KH
• Kim DS

• KMID: 2391456
• DOI: http://doi.org/10.3393/ac.2017.33.4.139

Abstract

PURPOSE
The anaphase-promoting complex (APC) is a multiprotein complex with E3 ubiquitin ligase activity and is required for ubiquitination of securin and cyclin-B. Several APC-targeting molecules are reported to be oncogenes. Dysregulation of APC may be associated with tumorigenesis. This study examines the relationship between APC expression and clinicopathological factors and evaluates the possibility of an aberrant APC function in colorectal carcinomas (CRCs).
METHODS
To determine whether the loss of APC7 expression is related to tumorigenesis, we used tissue micro-arrays in 114 resected CRCs to scrutinize the expressions of APC7 and Ki-67 immunohistochemistry and to find relations with clinocopathologic parameters. The expression of APC7 was defined as positive for summed scores of staining intensities from 0 to 3+.
RESULTS
Forty-four cases (67.7%) of colon cancer and 38 cases (77.6%) of rectal cancer showed immunopositive reactions to APC. The grade of APC expression was not statistically correlated with tumor location, age, T or TNM stage, or differentiation. However, the expression of APC did correlate with the expression of Ki-67 and to the tumor recurrent. Higher APC expression showed the better 5-year overall survival rate in 74% of grades 2, 3 groups (high expression) than 57% of grades 0, 1 groups (lower expression) respectively (P = 0.042).
CONCLUSION
Positive APC expression may be a good prognostic factor for patients with CRC, and the loss of APC expression in tumor tissue may be related with the risk for recurrence and a poor survival rate compared to high APC expression. Further study of APC in controlling the cell cycle as aberrant function in CRC is needed.

Keyword

Anaphase-promoting complex-cyclosome; Adenocarcinoma; Colorectal neoplasms; Cell cycle

MeSH Terms

Adenocarcinoma
Anaphase-Promoting Complex-Cyclosome
Carcinogenesis
Cell Cycle
Colonic Neoplasms
Colorectal Neoplasms*
Humans*
Immunohistochemistry
Oncogenes
Rectal Neoplasms
Recurrence
Securin
Survival Rate
Ubiquitin
Ubiquitin-Protein Ligases
Ubiquitination
Ubiquitin
Ubiquitin-Protein Ligases