Biomol Ther.  2014 Sep;22(5):420-425. 10.4062/biomolther.2014.066.

Protective Effects of Chlorogenic Acid against Experimental Reflux Esophagitis in Rats

• Kang JW
• Lee SM

• KMID: 2380319
• DOI: http://doi.org/10.4062/biomolther.2014.066

Abstract

Esophageal reflux of gastric contents causes esophageal mucosal damage and inflammation. Recent studies show that oxygen-derived free radicals mediate mucosal damage in reflux esophagitis (RE). Chlorogenic acid (CGA), an ester of caffeic acid and quinic acid, is one of the most abundant polyphenols in the human diet and possesses anti-inflammatory, antibacterial and anti-oxidant activities. In this context, we investigated the effects of CGA against experimental RE in rats. RE was produced by ligating the transitional region between the forestomach and the glandular portion and covering the duodenum near the pylorus ring with a small piece of catheter. CGA (10, 30 and 100 mg/kg) and omeprazole (positive control, 10 mg/kg) were administered orally 48 h after the RE operation for 12 days. CGA reduced the severity of esophageal lesions, and this beneficial effect was confirmed by histopathological observations. CGA reduced esophageal lipid peroxidation and increased the reduced glutathione/oxidized glutathione ratio. CGA attenuated increases in the serum level of tumor necrosis factor-alpha, and expressions of inducible nitric oxide synthase and cyclooxygenase-2 protein. CGA alleviates RE-induced mucosal injury, and this protection is associated with reduced oxidative stress and the anti-inflammatory properties of CGA.

Keyword

Chlorogenic acid; Gastroesophageal reflux disease; Inflammation; Oxidative stress; Reflux esophagitis

MeSH Terms

Animals
Catheters
Chlorogenic Acid*
Cyclooxygenase 2
Diet
Duodenum
Esophagitis, Peptic*
Free Radicals
Gastroesophageal Reflux
Glutathione
Humans
Inflammation
Lipid Peroxidation
Nitric Oxide Synthase Type II
Omeprazole
Oxidative Stress
Polyphenols
Pylorus
Quinic Acid
Rats*
Tumor Necrosis Factor-alpha
Chlorogenic Acid
Cyclooxygenase 2
Free Radicals
Glutathione
Nitric Oxide Synthase Type II
Omeprazole
Polyphenols
Quinic Acid
Tumor Necrosis Factor-alpha