Tissue Eng Regen Med.  2017 Apr;14(2):143-151. 10.1007/s13770-017-0025-6.

Isolation and Characterization of Human Chorionic Membranes Mesenchymal Stem Cells and Their Neural Differentiation

• Miao Z
• Sun H
• Xue Y

• KMID: 2377344
• DOI: http://doi.org/10.1007/s13770-017-0025-6

Abstract

Mesenchymal stem cells (MSCs) can be obtained from a variety of human tissues. Placenta has become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to localize and characterize MSCs within human chorionic membranes (hCMSCs). For this purpose, immunofluorescence labeling with CD105 and CD90 were used to determine the distribution of MSCs in chorionic membranes tissue. A medium supplemented with a synthetic serum and various concentrations of neurotrophic factors and cytokines was used to induce hCMSCs to neural cells. The results showed that the CD90 positive cells were scattered in the chorionic membranes tissue, and the CD105 positive cells were mostly located around the small blood vessels. hCMSCs expressed typical mesenchymal markers (CD73, CD90, CD105, CD44 and CD166) but not hematopoietic markers (CD45, CD34) and HLA-DR. hCMSCs differentiated into adipocytes, osteocytes, chondrocytes, and neuronal cells, as revealed by morphological changes, cell staining, immunofluorescence analyses, and RT-PCR showing the tissue-specific gene presence for differentiated cell lineages after the treatment with induce medium. Human chorionic membranes may be the source of MSCs for treatment of nervous system injury.

Keyword

Mesenchymal stem cells; Chorionic membranes; Neural differentiation; Hematopoietic markers

MeSH Terms

Adipocytes
Blood Vessels
Cell Lineage
Chondrocytes
Chorion*
Cytokines
Fluorescent Antibody Technique
HLA-DR Antigens
Humans*
Membranes*
Mesenchymal Stromal Cells*
Nerve Growth Factors
Neurons
Osteocytes
Placenta
Regenerative Medicine
Stem Cells
Tissue Engineering
Trauma, Nervous System
Cytokines
HLA-DR Antigens
Nerve Growth Factors