Nat Prod Sci.  2017 Mar;23(1):53-60. 10.20307/nps.2017.23.1.53.

Ethanol Extract of Perillae Herba Enhances Pentobarbital-Induced Sleep and Non-Rapid Eye Movement (NREM) Sleep through GABA(A)-ergic Systems

Affiliations
  • 1College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea. kiwan@chungbuk.ac.kr

Abstract

Perillae Herba has been traditionally used for the sedation in the oriental countries. Therefore, this study was conducted to determine whether Perillae Herba ethanol extract (PHEE) enhances pentobarbital-induced sleeping behaviors in animals. In addition, the possible mechanisms are demonstrated. PHEE (12.5, 25 and 50 mg/kg. p.o.) reduced the locomotor activity in mice. PHEE reduced sleep latency and augmented the total sleep time in pentobarbital (42 mg/kg, i.p.)-induced sleep in mice. Furthermore, the number of sleeping mice treated with sub-hypnotic pentobarbital (28 mg/kg, i.p.) increased. PHEE (50 mg/kg. p.o.) decreased the sleep/wake cycles and wakefulness, and increased total sleeping time and NREM sleep in electroencephalogram (EEG) of rats. In addition, PHEE (0.1, 1.0 and 10 µg/ml) increased the intracellular Cl⁻ level through the GABA receptors in the hypothalamus of rats. Moreover, the protein of glutamate decarboxylase (GAD) was overexpressed by PFEE. It was found that PHEE enhanced pentobarbital-induced sleeping behaviors through GABA(A)-ergic transmissions.

Keyword

Perillae Herba ethanol extract (PHEE); Pentobarbital-induced sleep; Glutamic acid decarboxylase (GAD); GABA; GABAA receptors; Electroencephalogram (EEG)

MeSH Terms

Animals
Electroencephalography
Ethanol*
Eye Movements*
gamma-Aminobutyric Acid
Glutamate Decarboxylase
Hypothalamus
Mice
Motor Activity
Pentobarbital
Perilla*
Rats
Receptors, GABA
Wakefulness
Ethanol
Glutamate Decarboxylase
Pentobarbital
Receptors, GABA
gamma-Aminobutyric Acid
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