Tissue Eng Regen Med.  2016 Oct;13(5):547-553. 10.1007/s13770-016-9127-9.

Hypoxia Enhances Osteogenic Differentiation in Retinoic Acid-Treated Murine-Induced Pluripotent Stem Cells

Affiliations
  • 1Mineralized Tissue Research Unit and Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand. thanaphum.o@chula.ac.th prasit215@gmail.com
  • 2Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Sendai, Japan.

Abstract

Hypoxic condition influences biological responses in various cell types. However, a hypoxic regulating osteogenic differentiation remains controversy. Here, an influence of short-term culture in hypoxic condition on osteogenic marker gene expression by retinoic acid-treated murine gingival fibroblast-derived induced pluripotent stem cells (RA-miPS) was investigated. Results demonstrated that hypoxic condition significantly upregulated Vegf, Runx2, Osx, and Ocn mRNA expression by RA-miPS in normal culture medium at day 3. Further, desferrioxamine significantly downregulated pluripotent marker (Nanog and Oct4) and enhanced osteogenic marker (Runx2, Osx, Dlx5, and Ocn) gene expression as well as promoted in vitro mineral deposition. However, the effect of cobalt chloride on osteogenic differentiation of RA-miPS was not robust. In summary, the results imply that hypoxic condition may be useful in the enhancement of osteogenic differentiation in RA-miPS.

Keyword

Pluripotent stem cells; Hypoxia; Desferrioxamine; Cobalt chloride

MeSH Terms

Anoxia*
Cobalt
Deferoxamine
Gene Expression
In Vitro Techniques
Induced Pluripotent Stem Cells
Miners
Pluripotent Stem Cells*
RNA, Messenger
Vascular Endothelial Growth Factor A
Cobalt
Deferoxamine
RNA, Messenger
Vascular Endothelial Growth Factor A
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