Ann Dermatol.  1996 Apr;8(2):90-97. 10.5021/ad.1996.8.2.90.

Effects of Interleukin-4 on Extracellular Matrix Gene Expression in Normal Skin Fibroblasts Cultures

• Kim TH
• Kim SW
• Kwon HJ
• Ryoo YW
• Kim BC
• Lee KS

• KMID: 2360537
• DOI: http://doi.org/10.5021/ad.1996.8.2.90

Abstract

BACKGROUND
Interleukin-4(IL-4) is a 20 kDa glycoprotein and is now known to possess fibrogenic activities.
OBJECTIVE
The purpose of this study is to evaluate the effect of IL4 on the extracellular matrix gene expression.
METHODS
Quantitation of collagenous protein synthesis, Northern and dot-blot hybridization, transfection experiments and CAT assay in normal human skin fibroblasts were done.
RESULTS
Maximal elevation of collagen synthesis was presented at the concentration of IL-4 being S.Ong/ml. In Northern and dot-blot analysis, each level of type I collagens and fibronectin mRNA increased 3.0, and 2.8-fold, respectively in IL4 treated fibroblasts. In CAT to c assay, the percentage of acetylation was 8.3% in the untreated control group and 23.1% in 5.Ong/ml of the IL-4 treated group in normal fibroblasts. The promoter activity was in creased 2.8-fold in II.4(5.Ong/ml) treated fibroblasts compared to the control groups.
CONCLUSION
IL4 might be a fibrogenic cytokine that could be important in promoting the biogenesis of collagen proteins. This could be due to increased transcription.

Keyword

Interleukin-4; Extracellular matrix genes; Fibroblast

MeSH Terms

Acetylation
Animals
Cats
Collagen
Collagen Type I
Extracellular Matrix*
Fibroblasts*
Fibronectins
Gene Expression*
Glycoproteins
Humans
Interleukin-4*
RNA, Messenger
Skin*
Transfection
Collagen
Collagen Type I
Fibronectins
Glycoproteins
Interleukin-4
RNA, Messenger