Ann Dermatol.  1995 Oct;7(4):303-307. 10.5021/ad.1995.7.4.303.

Expression of the Extracellular Matrix Gene in Response to Insulin and Insulin-like Growth Factor in Cultured Skin Fibroblasts

• Kwon HJ
• Kim BC
• Lee KS

• KMID: 2361358
• DOI: http://doi.org/10.5021/ad.1995.7.4.303

Abstract

BACKGROUND
Diabetes mellitus is a heterogeneous group of disorders characterized by high serum glucose levels and by disturbances of carbohydrate and lipid metabolism. There are many cutaneous signs of this common endocrinopathy, such as nercobiosis lipoidica diabeticorum, diabetic bullosis, shin spot, diabetic pruritus, etc.
OBJECTIVE
In this study, we investigated whether extracellular matrix gene expression in cultured skin fibroblast is influenced by insulin and Insulin-like growth factor-I(IGF-I). METHOD: Total RNA was isolated from insulin or IGF-I treated human skin fibroblasts. The Northern blot and slot-blot hybridization were then conducted.
RESULTS
The mRNA levels of pro α1(I) collagen, pro α1(I11) collagen, fibronectin in insulin and IGF-I treated normal skin fibroblasts increased compared with untreated normal skin fibroblasts.
CONCLUSION
Our results show that insulin and IGF-I stimulate collagen formation in normal skin fibroblast at physiological concentrations. Therefore, these demonstrate that insulin can modulate the expression of extracellular matrix gene.

Keyword

Diabetes mellitus; Insulin; Insulin-like growth factor; Extracellular matrix gene

MeSH Terms

Blood Glucose
Blotting, Northern
Collagen
Diabetes Mellitus
Extracellular Matrix*
Fibroblasts*
Fibronectins
Gene Expression
Humans
Insulin*
Insulin-Like Growth Factor I
Lipid Metabolism
Methods
Pruritus
RNA
RNA, Messenger
Skin*
Collagen
Fibronectins
Insulin
Insulin-Like Growth Factor I
RNA
RNA, Messenger